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验证数据展示

  • IF Staining of HeLa using 83174-5-RR

    IF Staining of HeLa using 83174-5-RR

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using PRKD1 antibody (83174-5-RR, Clone: 230355D12 ) at dilution of 1:100 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • IF Staining of LNCaP using 83174-5-RR

    IF Staining of LNCaP using 83174-5-RR

    Immunofluorescent analysis of (-20°C Ethanol) fixed LNCaP cells using PRKD1 antibody (83174-5-RR, Clone: 230355D12 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • FC experiment of HeLa using 83174-5-RR

    FC experiment of HeLa using 83174-5-RR

    1x10^6 HeLa cells were intracellularly stained with 0.25 ug Prkd1 Recombinant Antibody (83174-5-RR, Clone:230355D12) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

PRKD1 Recombinant antibody

PRKD1 Uni-rAbTM Recombinant Antibody for IF/ICC, FC (Intra), ELISA
Cat No. 83174-5-RR

产品说明书

CloneNo. 230355D12

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

IF/ICC, FC (Intra), ELISA

别名

Serine/threonine protein kinase D1, PRKCM, PKD1, PKD, 230355D12

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive IF/ICC detected inHeLa cells, LNCaP cells
Positive FC (Intra) detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy PRKD1 Ready-To-Use IHC Kit. PRKD1 primary antibody included.

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

83174-5-RR targets PRKD1 in IF/ICC, FC (Intra), ELISA applications and shows reactivity with human samples.

经测试应用 IF/ICC, FC (Intra), ELISA Application Description
经测试反应性 human
免疫原 Peptide 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 protein kinase D1
别名 Serine/threonine protein kinase D1, PRKCM, PKD1, PKD, 230355D12
计算分子量 102 kDa
GenBank蛋白编号NM_002742
基因名称 PRKD1
Gene ID (NCBI) 5587
RRIDAB_3670868
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purfication
UNIPROT IDQ15139
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
IF protocol for PRKD1 antibody 83174-5-RRDownload protocol
FC protocol for PRKD1 antibody 83174-5-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols