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N4BP3 Recombinant antibody

N4BP3 Uni-rAbTM Recombinant Antibody for WB, IHC, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

230547D12

Cat no : 83178-2-RR

Print datasheet

Synonyms

NEDD4-binding protein 3, Nedd4 binding protein 3, KIAA0341, 230547D12

验证数据展示

  • FC experiment of MCF-7 using 83178-2-RR

    FC experiment of MCF-7 using 83178-2-RR

    1x10^6 MCF-7 cells were intracellularly stained with 0.25 ug N4BP3 Recombinant antibody (83178-2-RR, Clone:230547D12) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

  • WB analysis of MCF-7 using 83178-2-RR

    WB analysis of MCF-7 using 83178-2-RR

    MCF-7 cells were subjected to SDS PAGE followed by western blot with 83178-2-RR (N4BP3 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IHC staining of human liver using 83178-2-RR

    IHC staining of human liver using 83178-2-RR

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 83178-2-RR (N4BP3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human liver using 83178-2-RR

    IHC staining of human liver using 83178-2-RR

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 83178-2-RR (N4BP3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Affinity and Kinetic Characterization of 83178-2-RR

    Affinity and Kinetic Characterization of 83178-2-RR

    Biolayer interferometry (BLl) kinetic assays of 83178-2-RR against Human N4BP3 were performed. The affinity constant is 66.3 pM.

经过测试的应用

Positive WB detected inMCF-7 cells
Positive IHC detected inhuman liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive FC (Intra) detected inMCF-7 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

83178-2-RR targets N4BP3 in WB, IHC, FC (Intra), ELISA applications and shows reactivity with human samples.

Tested Applications WB, IHC, FC (Intra), ELISA Application Description
Tested Reactivity human
Immunogen N4BP3 fusion protein Ag10194 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name Nedd4 binding protein 3
Synonyms NEDD4-binding protein 3, Nedd4 binding protein 3, KIAA0341, 230547D12
Calculated Molecular Weight 544 aa, 60 kDa
Observed Molecular Weight 65-70 kDa
GenBank Accession NumberBC053323
Gene Symbol N4BP3
Gene ID (NCBI) 23138
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDO15049
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

N4BP3, also named as KIAA0341, a Fezzin family member, during nerve cell development. It is crucial for axonal and dendritic branching in developing neurons. It binds the protein NEDD4 and interacts with 14-3-3 proteins. N4BP3 is present in early neurites, growth cones, axons and dendrites of developing primary hippocampal neurons.

实验方案

Product Specific Protocols
WB protocol for N4BP3 antibody 83178-2-RRDownload protocol
IHC protocol for N4BP3 antibody 83178-2-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols