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SLC22A4 Recombinant antibody

SLC22A4 Uni-rAbTM Recombinant Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

240064D5

Cat no : 83192-1-RR

Print datasheet

Synonyms

UT2H, OCTN1, ETTh, ETT, 240064D5

验证数据展示

  • WB analysis using 83192-1-RR

    WB analysis using 83192-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 83192-1-RR (SLC22A4 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Affinity and Kinetic Characterization of 83192-1-RR

    Affinity and Kinetic Characterization of 83192-1-RR

    Biolayer interferometry (BLl) kinetic assays of 83192-1-RR against Human SLC22A4 were performed. The affinity constant is 1.08 nM.

经过测试的应用

Positive WB detected inmouse liver tissue, mouse kidney tissue

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

83192-1-RR targets SLC22A4 in WB, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications WB, ELISA Application Description
Tested Reactivity human, mouse
Immunogen SLC22A4 fusion protein Ag3974 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name solute carrier family 22 (organic cation/ergothioneine transporter), member 4
Synonyms UT2H, OCTN1, ETTh, ETT, 240064D5
Calculated Molecular Weight 551 aa, 62 kDa
Observed Molecular Weight 62-65 kDa
GenBank Accession NumberBC028313
Gene Symbol SLC22A4
Gene ID (NCBI) 6583
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ9H015
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

SLC22A4 (solute carrier family 22 member 4), also known as OCTN1. It is expected to be located in cell membrane and mitochondrion membrane. It is strongly expressed in kidney, trachea, bone marrow and fetal liver and in several human cancer cell lines, but not in adult liver (PMID: 9426230). The protein functions as a Na+-dependent and pH-dependent high affinity microbial symporter of potent food-derived antioxidant ergothioeine (PubMed:15795384), which transports one sodium ion with one ergothioeine molecule.

实验方案

Product Specific Protocols
WB protocol for SLC22A4 antibody 83192-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols