验证数据展示
经过测试的应用
| Positive WB detected in | HEK-293T cells, HeLa cells, A549 cells, Jurkat cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
83206-1-RR targets GRAF in WB, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, ELISA Application Description |
| 经测试反应性 | human |
| 免疫原 | GRAF fusion protein Ag12008 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Recombinant |
| 产品类型 | Antibody |
| 全称 | Rho GTPase activating protein 26 |
| 别名 | ARHGAP26, 240086A11, Oligophrenin-1-like protein, Oligophrenin 1 like protein, KIAA0621 |
| 计算分子量 | 759 aa, 86 kDa |
| 观测分子量 | 92-100 kDa |
| GenBank蛋白编号 | BC068555 |
| 基因名称 | ARHGAP26 |
| Gene ID (NCBI) | 23092 |
| RRID | AB_3670894 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | Q9UNA1 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
GTPase Regulator Associated with Focal Adhesion Kinase (GRAF), also known as Rho GTPase activating protein 26 (ARHGAP26) , is a GTPase-activating protein and inhibits the activity of Rho GTPases by promoting the hydrolytic ability of Rho GTPases. GRAF enhances the hydrolysis of GTPases and converts GTPases from an active form to an inactive form, thereby inhibiting signaling transduction. Deletion and mutation of ARHGAP26 can lead to promyelocytic leukemia, suggesting tumor suppressive activity of ARHGAP26. ARHGAP26 was downregulated in glioblastoma and associated with cell proliferation and migration (PMID: 10908648, PMID: 17611651, PMID: 31004081).
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for GRAF antibody 83206-1-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |