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nectin 4 Recombinant antibody, PBS Only (Detector)

nectin 4 Recombinant Antibody for Indirect ELISA, Cytometric bead array

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

Indirect ELISA, Cytometric bead array

Conjugate

Unconjugated

CloneNo.

240009F8

Cat No : 83223-3-PBS

Print datasheet

Synonyms

Ig superfamily receptor LNIR, LNIR, nectin 4, poliovirus receptor related 4, PRR4, PVRL4

验证数据展示

  • Cytometric bead array standard curve of MP00147-2

    Cytometric bead array standard curve of MP00147-2

    Cytometric bead array standard curve of MP00147-2, nectin 4 Recombinant Matched Antibody Pair - PBS only. Capture antibody: 83223-1-PBS. Detection antibody: 83223-3-PBS. Standard: Eg0415. Range: 0.625-10 ng/mL.

Planning an IHC experiment? We recommend our IHCeasy Nectin-4/PVRL4 Ready-To-Use IHC Kit. Nectin-4/PVRL4 primary antibody included.

产品信息

83223-3-PBS targets nectin 4 as part of a matched antibody pair:

MP00147-2: 83223-1-PBS capture and 83223-3-PBS detection (validated in Cytometric bead array)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Applications Indirect ELISA, Cytometric bead array Application Description
Tested Reactivity Human
Immunogen Recombinant protein 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Affinity
KD=5.81 x 10-10M
KOff=1.47 x 10-4M
KOn=2.53 x 105M
Full Name poliovirus receptor-related 4
Synonyms Ig superfamily receptor LNIR, LNIR, nectin 4, poliovirus receptor related 4, PRR4, PVRL4
Calculated Molecular Weight 510 aa, 55 kDa
GenBank Accession NumberBC010423
Gene Symbol Nectin-4
Gene ID (NCBI) 81607
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ96NY8
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Nectin-4, also known as PVRL4, is a type-I transmembrane glycoprotein that belongs to the nectin subfamily of immunoglobulin-like adhesion molecules that participate in Ca(2+)-independent cell-cell adhesion (PMID: 18703497). The extracellular domain of Nectin-4, which contains three immunoglobulin-like domains (V and two C2-type domains, VCC), can be proteolytically cleaved to release a soluble form. Nectin-4 interacts with afadin via its carboxyl-terminal cytoplasmic sequence and trans-interacts with nectin-1/PRR1 through V domain interaction (PMID: 11544254). It acts as a receptor for measles virus (PMID: 22048310). Nectin-4 is overexpressed in multiple human malignancies and the aberrant expression is correlated with cancer progression and poor prognostic (PMID: 35131876). Nectin-4 can be detected as a soluble form of 43 kDa and a transmembrane form of 66 kDa (PMID: 15784625).