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Mouse HO-1/Hmox1 Recombinant antibody, PBS Only (Detector)

HO-1/Hmox1 Uni-rAbTM Recombinant Antibody for Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Host / Isotype

Rabbit / IgG

Reactivity

mouse

Applications

Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Conjugate

Unconjugated

CloneNo.

240236G6

Cat no : 83291-2-PBS

Print datasheet

Synonyms

Hmox1, HO-1, Hmox, Hemox, heme oxygenase (decycling) 1

验证数据展示

  • Sandwich ELISA standard curve of MP00318-3

    Sandwich ELISA standard curve of MP00318-3

    Sandwich ELISA standard curve of MP00318-3, mouse HO-1/Hmox Recombinant Matched Antibody Pair - PBS only. 83291-4-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard: Eg0938. 83291-2-PBS was HRP conjugated as the detection antibody. Range: 78.1-5000 pg/mL.

  • Sample test of MP00318-3

    Sample test of MP00318-3

    Mouse spleen tissue (3 spleen in 1-2 mm pieces in 100 mL of medium) was cultured in RPMI supplemented with 10% fetal bovine serum, 50 μM β-mercaptoethanol, 2 mM L-glutamine, 100 U/mL penicillin, and 100 μg/mL streptomycin sulfate. An aliquot of the cell culture supernatant was removed, assayed for mouse HO-1/Hmox1,and measured 225.2 pg/mL.

  • Sample test of MP00318-3

    Sample test of MP00318-3

    The mean mouse HO-1/Hmox1 concentration was determined to be 23,262.0 pg/mL in NIH/3T3 cell extract based on a 7.8 mg/mL extract load and 41,977.1 pg/mL in mouse liver extract based on a 5 mg/mL extract load.

  • Sample test of MP00318-3

    Sample test of MP00318-3

    Serum of sixteen mice was measured. The mouse HO-1/Hmox1 concentration of detected samples was determined to be 547.1 pg/mL with a range of 297.1 - 1,158.6 pg/mL.

  • Cytometric bead array standard curve of MP00318-1

    Cytometric bead array standard curve of MP00318-1

    Cytometric bead array standard curve of MP00318-1, MOUSE HO-1/Hmox1 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83291-1-PBS. Detection antibody: 83291-2-PBS. Standard: Eg0938. Range: 1.25-20 ng/mL.

  • Cytometric bead array standard curve of MP00318-2

    Cytometric bead array standard curve of MP00318-2

    Cytometric bead array standard curve of MP00318-2, MOUSE HO-1/Hmox1 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83291-3-PBS. Detection antibody: 83291-2-PBS. Standard: Eg0938. Range: 1.25-20 ng/mL.

产品信息

83291-2-PBS targets HO-1/Hmox1 as part of a matched antibody pair:

MP00318-1: 83291-1-PBS capture and 83291-2-PBS detection (validated in Cytometric bead array)

MP00318-2: 83291-3-PBS capture and 83291-2-PBS detection (validated in Cytometric bead array)

MP00318-3: 83291-4-PBS capture and 83291-2-PBS detection (validated in Sandwich ELISA)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Applications Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test Application Description
Tested Reactivity mouse
Immunogen Fusion Protein 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name heme oxygenase (decycling) 1
Synonyms Hmox1, HO-1, Hmox, Hemox, heme oxygenase (decycling) 1
Calculated Molecular Weight33 kDa
GenBank Accession NumberNM_010442.2
Gene Symbol HO-1
Gene ID (NCBI) 15368
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP14901
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C