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Conjugate
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验证数据展示

  • WB analysis of RAW 264.7 using 83297-8-RR

    WB analysis of RAW 264.7 using 83297-8-RR

    RAW 264.7 cells were subjected to SDS PAGE followed by western blot with 83297-8-RR (CD14 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IF Staining of RAW 264.7 using 83297-8-RR

    IF Staining of RAW 264.7 using 83297-8-RR

    Immunofluorescent analysis of (4% PFA) fixed RAW 264.7 cells using CD14 antibody (83297-8-RR, Clone: 240225C2 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • Affinity and Kinetic Characterization of 83297-8-RR

    Affinity and Kinetic Characterization of 83297-8-RR

    Biolayer interferometry (BLl) kinetic assays of 83297-8-RR against Mouse CD14 were performed. The affinity constant is 0.219 nM.

CD14 Recombinant antibody

CD14 Uni-rAbTM Recombinant Antibody for WB, IF/ICC, ELISA
Cat No. 83297-8-RR

产品说明书

CloneNo. 240225C2

宿主/亚型

Rabbit / IgG

种属反应性

mouse

应用

WB, IF/ICC, ELISA

别名

CD14 antigen, 240225C2

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inRAW 264.7 cells
Positive IF/ICC detected inRAW 264.7 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)/ICCIF/ICC : 1:125-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

83297-8-RR targets CD14 in WB, IF/ICC, ELISA applications and shows reactivity with mouse samples.

经测试应用 WB, IF/ICC, ELISA Application Description
经测试反应性 mouse
免疫原 Fusion Protein 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 CD14 antigen
别名 CD14 antigen, 240225C2
计算分子量39 kDa
观测分子量45-55 kDa
GenBank蛋白编号NM_009841.4
基因名称 Cd14
Gene ID (NCBI) 12475
RRIDAB_3670967
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purfication
UNIPROT IDP10810
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CD14 is a 50-55 kDa glycosylphosphatidylinositol-anchored glycoprotein preferentially expressed on monocytes and macrophages, and at lower levels on granulocytes (PMID: 3385210; 2462937; 7685797). CD14 can also exist as a soluble protein. CD14 acts as a co-receptor for bacterial liposaccharides (LPS) (PMID: 1698311). It plays a major role in the inflammatory response of monocytes to LPS.

实验方案

Product Specific Protocols
WB protocol for CD14 antibody 83297-8-RRDownload protocol
IF protocol for CD14 antibody 83297-8-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols