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STX1B Recombinant antibody

STX1B Recombinant Antibody for WB, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, mouse

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

240156B9

Cat No : 83298-1-RR

Print datasheet

Synonyms

STX1B, STX1B1, STX1B2, syntaxin 1B, Syntaxin 1B1, Syntaxin 1B2

验证数据展示

  • WB analysis of Y79 using 83298-1-RR

    WB analysis of Y79 using 83298-1-RR

    Y79 cells were subjected to SDS PAGE followed by western blot with 83298-1-RR (STX1B antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • IF Staining of mouse brain using 83298-1-RR

    IF Staining of mouse brain using 83298-1-RR

    Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using STX1B antibody (83298-1-RR, Clone: 240156B9 ) at dilution of 1:1000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • Affinity experiments of 83298-1-RR

    Affinity experiments of 83298-1-RR

    Biolayer interferometry (BLl) kinetic assays of 83298-1-RR against Human STX1B were performed. The affinity constant is below 1 pM.

经过测试的应用

Positive WB detected inY79 cells
Positive IF detected inmouse brain tissue
Planning an IHC experiment? We recommend our IHCeasy Syntaxin 1A/1B Ready-To-Use IHC Kit. Syntaxin 1A/1B primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)IF : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

The immunogen of 83298-1-RR is STX1B Fusion Protein expressed in E. coli.

Tested Applications WB, IF/ICC, ELISA Application Description
Tested Reactivity Human, mouse
Immunogen STX1B fusion protein Ag10275 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name syntaxin 1B
Synonyms STX1B, STX1B1, STX1B2, syntaxin 1B, Syntaxin 1B1, Syntaxin 1B2
Calculated Molecular Weight 288 aa, 33 kDa
Observed Molecular Weight 33-35 kDa
GenBank Accession NumberBC062298
Gene Symbol STX1B
Gene ID (NCBI) 112755
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP61266
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Syntaxins are a family of transmembrane proteins that belong to the SNARE complex (PMID: 11737951). In conjunction with other SNAREs and with the cytoplasmic NSF and SNAP proteins, syntaxins mediate vesicle fusion in diverse vesicular transport processes along the exocytic and the endocytic pathway (PMID: 11737951). Syntaxin 1A and 1B, two closely related isoforms of syntaxin 1, are involved in synaptic vesicle docking and fusion during neurotransmitter release (PMID: 7690687; 1321498; 18691641).

实验方案

Product Specific Protocols
WB protocol for STX1B antibody 83298-1-RRDownload protocol
IF protocol for STX1B antibody 83298-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols