SMARCA4 Recombinant antibody
SMARCA4 Recombinant Antibody for WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
Human, rat, mouse
Applications
WB, ELISA
Conjugate
Unconjugated
CloneNo.
240172F7
验证数据展示
经过测试的应用
Positive WB detected in | HeLa cells, HepG2 cells, HSC-T6 cells, NIH/3T3 cells, HEK-293T cells, C6 cells |
推荐稀释比
Application | Dilution |
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Western Blot (WB) | WB : 1:5000-1:50000 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
The immunogen of 83310-7-RR is SMARCA4 Fusion Protein expressed in E. coli.
Tested Applications | WB, ELISA Application Description |
Tested Reactivity | Human, rat, mouse |
Immunogen | SMARCA4 fusion protein Ag16256 种属同源性预测 |
Host / Isotype | Rabbit / IgG |
Class | Recombinant |
Type | Antibody |
Full Name | SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily a, member 4 |
Synonyms | ATP dependent helicase SMARCA4, BAF190, BAF190A, BRG1, BRG1 associated factor 190A, FLJ39786, hSNF2b, Protein brahma homolog 1, Protein BRG 1, SMARCA4, SNF2, SNF2 BETA, SNF2B, SNF2L4, SNF2LB, SWI2, Transcription activator BRG1 |
Calculated Molecular Weight | 1647 aa, 185 kDa |
Observed Molecular Weight | 185 kDa |
GenBank Accession Number | BC150298 |
Gene Symbol | SMARCA4 |
Gene ID (NCBI) | 6597 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Protein A purfication |
UNIPROT ID | P51532 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
SMARCA4, also named as BAF190A, BRG1, SNF2B and SNF2L4, belongs to the SNF2/RAD54 helicase family. SMARCA4 is a transcriptional coactivator cooperating with nuclear hormone receptors to potentiate transcriptional activation. It is a component of the CREST-BRG1 complex, a multiprotein complex that regulates promoter activation by orchestrating a calcium-dependent release of a repressor complex and a recruitment of an activator complex. It is also involved in vitamin D-coupled transcription regulation via its association with the WINAC complex, a chromatin-remodeling complex recruited by vitamin D receptor (VDR), which is required for the ligand-bound VDR-mediated transrepression of the CYP27B1 gene.
实验方案
Product Specific Protocols | |
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WB protocol for SMARCA4 antibody 83310-7-RR | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |