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  • Featured Product
  • KD/KO Validated

GANAB Recombinant antibody, PBS Only (Capture)

GANAB Uni-rAbTM Recombinant Antibody for WB, IHC, IF/ICC, FC (Intra), Cytometric bead array, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, FC (Intra), Cytometric bead array, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

240279B7

Cat no : 83324-1-PBS

Print datasheet

Synonyms

Glucosidase II subunit alpha, G2AN, EC:3.2.1.207, Alpha-glucosidase 2, Alpha glucosidase 2

验证数据展示

  • WB analysis using 83324-1-RR (same clone as 83324-1-PBS)

    WB analysis using 83324-1-RR (same clone as 83324-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 83324-1-RR (GANAB antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83324-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse liver using 83324-1-RR (same clone as 83324-1-PBS)

    IHC staining of mouse liver using 83324-1-RR (same clone as 83324-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 83324-1-RR (GANAB antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83324-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with GANAB antibody included
  • FC experiment of HeLa using 83324-1-RR (same clone as 83324-1-PBS)

    FC experiment of HeLa using 83324-1-RR (same clone as 83324-1-PBS)

    1x10^6 HeLa cells were intracellularly stained with 0.25 ug GANAB Recombinant antibody (83324-1-RR, Clone:240279B7)(red) or Isotype Controland (blue) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 83324-1-PBS in a different storage buffer formulation.

  • WB analysis of HeLa using 83324-1-RR (same clone as 83324-1-PBS)

    WB analysis of HeLa using 83324-1-RR (same clone as 83324-1-PBS)

    WB result of GANAB antibody (83324-1-RR; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-GANAB transfected HeLa cells. This data was developed using the same antibody clone with 83324-1-PBS in a different storage buffer formulation.

  • IF Staining of HeLa using 83324-1-RR (same clone as 83324-1-PBS)

    IF Staining of HeLa using 83324-1-RR (same clone as 83324-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using GANAB antibody (83324-1-RR, Clone: 240279B7 ) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 83324-1-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP00350-1

    Cytometric bead array standard curve of MP00350-1

    Cytometric bead array standard curve of MP00350-1, GANAB Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83324-1-PBS. Detection antibody: 83324-2-PBS. Standard: Ag30417. Range: 1.25-40 ng/mL.

  • Affinity and Kinetic Characterization of 83324-1-PBS

    Affinity and Kinetic Characterization of 83324-1-PBS

    Biolayer interferometry (BLl) kinetic assay of 83324-1-PBS against Human GANAB was performed. The affinity constant is 0.16 nM.

  • Affinity and Kinetic Characterization of 83324-1-RR

    Affinity and Kinetic Characterization of 83324-1-RR

    Biolayer interferometry (BLl) kinetic assays of 83324-1-RR against Human GANAB were performed. The affinity constant is 0.186 nM.

Planning an IHC experiment? We recommend our IHCeasy GANAB Ready-To-Use IHC Kit. GANAB primary antibody included.

产品信息

83324-1-PBS targets GANAB as part of a matched antibody pair:

MP00350-1: 83324-1-PBS capture and 83324-2-PBS detection (validated in Cytometric bead array)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Applications WB, IHC, IF/ICC, FC (Intra), Cytometric bead array, Indirect ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen GANAB fusion protein Ag30417 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name glucosidase, alpha; neutral AB
Synonyms Glucosidase II subunit alpha, G2AN, EC:3.2.1.207, Alpha-glucosidase 2, Alpha glucosidase 2
Calculated Molecular Weight944aa,107 kDa; 488aa,55 kDa
Observed Molecular Weight110 kDa
GenBank Accession NumberBC017435
Gene Symbol GANAB
Gene ID (NCBI) 23193
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ14697
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C