DNase I Recombinant antibody
DNase I Uni-rAbTM Recombinant Antibody for WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse
Applications
WB, ELISA
Conjugate
Unconjugated
CloneNo.
240222F11
验证数据展示
经过测试的应用
| Positive WB detected in | mouse heart tissue, human urine sample |
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
The immunogen of 83337-6-RR is DNase I Fusion Protein expressed in E. coli.
| Tested Applications | WB, ELISA Application Description |
| Tested Reactivity | human, mouse |
| Immunogen | DNase I fusion protein Ag10117 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Full Name | deoxyribonuclease I |
| Synonyms | DNASE1, EC:3.1.21.1, DRNI, DNL1, Deoxyribonuclease-1 |
| Calculated Molecular Weight | 282 aa, 31 kDa |
| Observed Molecular Weight | 31-40 kDa |
| GenBank Accession Number | BC029437 |
| Gene Symbol | DNASE1 |
| Gene ID (NCBI) | 1773 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purfication |
| UNIPROT ID | P24855 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Deoxyribonuclease I (DNase I), also known as DNL1 and DRNI, belongs to the DNase I family. DNase I is a DNA-digestive glycoprotein that cleaves both single- and double stranded DNA through hydrolysis of the phosphodiester bond. In humans, the DNase I enzyme is primarily secreted by the pancreas, parotid, and pituitary glands into the digestive system and bloodstream. Since DNase I is ubiquitously expressed in human tissues, it is thought to play a similar anti-autoimmune role in other organs as well (PMID: 21806320). The calculated molecular weight of DNase I is 31 kDa. Sometimes higher molecular weight around 40 kDa can also be observed, which is caused by glycosylation (PMID: 34329318).
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for DNase I antibody 83337-6-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
