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IGFBP1 Recombinant antibody, PBS Only (Detector)

IGFBP1 Uni-rAbTM Recombinant Antibody for Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Conjugate

Unconjugated

CloneNo.

240263H8

Cat no : 83347-4-PBS

Print datasheet

Synonyms

IBP-1, IBP1, IBP 1, hIGFBP 1, AFBP

验证数据展示

  • Cytometric bead array standard curve of MP00355-3

    Cytometric bead array standard curve of MP00355-3

    Cytometric bead array standard curve of MP00355-3, IGFBP1 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83347-2-PBS. Detection antibody: 83347-4-PBS. Standard: Eg0951. Range: 0.313-40 ng/mL.

  • Sandwich ELISA standard curve of MP00355-3

    Sandwich ELISA standard curve of MP00355-3

    Sandwich ELISA standard curve of MP00355-3, Human IGFBP1 Recombinant Matched Antibody Pair - PBS only. 83347-2-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Eg0951. 83347-4-PBS was HRP conjugated as the detection antibody. Range: 62.5-4000 pg/mL.

  • Sample test of MP00355-3

    Sample test of MP00355-3

    Serum of eight individual healthy human donors was measured. The IGFBP1 concentration of detected samples was determined to be 18,764.2 pg/mL with a range of 1,025.1 - 37,369.6 pg/mL.

  • Sample test of MP00355-3

    Sample test of MP00355-3

    HepG2 cells were cultured in DMEM supplemented with 10% fetal bovine serum, 2.5 mM L-glutamine, 100 U/mL penicillin, and 100 μg/mL streptomycin sulfate. An aliquot of the cell culture supernate was removed, assayed for human IGFBP1, and measured 43,726.4 pg/mL.

  • Sample test of MP00355-3

    Sample test of MP00355-3

    The mean IGFBP1 concentration was determined to be 38,698.4 pg/mL in HepG2 cell extract based on a 1.5 mg/mL extract load.

Planning an IHC experiment? We recommend our IHCeasy IGFBP1 Ready-To-Use IHC Kit. IGFBP1 primary antibody included.

产品信息

83347-4-PBS targets IGFBP1 as part of a matched antibody pair:

MP00355-3: 83347-2-PBS capture and 83347-4-PBS detection (validated in Cytometric bead array, Sandwich ELISA)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Applications Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test Application Description
Tested Reactivity human
Immunogen Fusion Protein 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name IGF binding protein 1
Synonyms IBP-1, IBP1, IBP 1, hIGFBP 1, AFBP
Calculated Molecular Weight28 kDa
GenBank Accession NumberNM_000596.4
Gene Symbol IGFBP1
Gene ID (NCBI) 3484
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP08833
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C