• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

验证数据展示

  • WB analysis using 83362-2-RR (same clone as 83362-2-PBS)

    WB analysis using 83362-2-RR (same clone as 83362-2-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 83362-2-RR (NEDD9 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83362-2-PBS in a different storage buffer formulation.

  • WB analysis using 83362-2-RR (same clone as 83362-2-PBS)

    WB analysis using 83362-2-RR (same clone as 83362-2-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 83362-2-RR (NEDD9 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83362-2-PBS in a different storage buffer formulation.

  • IF Staining of MCF-7 using 83362-2-RR (same clone as 83362-2-PBS)

    IF Staining of MCF-7 using 83362-2-RR (same clone as 83362-2-PBS)

    Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using NEDD9 antibody (83362-2-RR, Clone: 240310F8 ) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 83362-2-PBS in a different storage buffer formulation.

  • IF Staining of A549 using 83362-2-RR (same clone as 83362-2-PBS)

    IF Staining of A549 using 83362-2-RR (same clone as 83362-2-PBS)

    Immunofluorescent analysis of (4% PFA) fixed A549 cells using NEDD9 antibody (83362-2-RR, Clone: 240310F8 ) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-phalloidin (red). This data was developed using the same antibody clone with 83362-2-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP00378-2

    Cytometric bead array standard curve of MP00378-2

    Cytometric bead array standard curve of MP00378-2, NEDD9 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83362-2-PBS. Detection antibody: 83362-4-PBS. Standard: Ag34170. Range: 2.5-80 ng/mL.

  • Affinity and Kinetic Characterization of 83362-2-PBS

    Affinity and Kinetic Characterization of 83362-2-PBS

    Biolayer interferometry (BLl) kinetic assay of 83362-2-PBS against Human NEDD9 was performed. The affinity constant is 0.225 nM.

  • Affinity and Kinetic Characterization of 83362-2-RR

    Affinity and Kinetic Characterization of 83362-2-RR

    Biolayer interferometry (BLl) kinetic assays of 83362-2 against Human NEDD9 were performed. The affinity constant is 0.173 nM.

NEDD9 Recombinant antibody, PBS Only (Capture)

NEDD9 Uni-rAbTM Recombinant Antibody for WB, IF/ICC, Cytometric bead array, Indirect ELISA
Cat No. 83362-2-PBS
CloneNo. 240310F8

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, Cytometric bead array, Indirect ELISA

Synonyms

HEF1, Enhancer of filamentation 1 p55, CRK-associated substrate-related protein, CAS-L, CASL

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size: 

-/ -

点击咨询售前客服

点击咨询售后客服

产品信息

83362-2-PBS targets NEDD9 as part of a matched antibody pair:

MP00378-2: 83362-2-PBS capture and 83362-4-PBS detection (validated in Cytometric bead array)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Applications WB, IF/ICC, Cytometric bead array, Indirect ELISA Application Description
Tested Reactivity human
Immunogen NEDD9 fusion protein Ag34170 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name neural precursor cell expressed, developmentally down-regulated 9
Synonyms HEF1, Enhancer of filamentation 1 p55, CRK-associated substrate-related protein, CAS-L, CASL
Calculated Molecular Weight 834 aa, 93 kDa
Observed Molecular Weight 105 kDa, 115 kDa
GenBank Accession NumberBC040207
Gene Symbol NEDD9
Gene ID (NCBI) 4739
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ14511
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

NEDD9, also known as CASL, HEF1, contains an N-terminal Src homology (SH) 3 domain and an adjacent domain composed of multiple SH2-binding motifs(PMID: 8668148). Cell cycle-regulated processing produces four isoforms: p115, p105, p65, and p55. Isoform p115 arises from p105 phosphorylation and appears later in the cell cycle. Isoform p55 arises from p105 as a result of cleavage at a caspase cleavage-related site and it appears specifically at mitosis. p105 and p115 are predominantly cytoplasmic and associated with focal adhesions, whereas p55associates with the mitotic spindle(PMID: 9584194).