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MTHFR Recombinant antibody, PBS Only (Capture)

MTHFR Uni-rAbTM Recombinant Antibody for Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Conjugate

Unconjugated

CloneNo.

240348C3

Cat no : 83372-5-PBS

Print datasheet

Synonyms

Methylenetetrahydrofolate reductase (NADPH), EC:1.5.1.53, 240348C3

验证数据展示

  • Sandwich ELISA standard curve of MP00372-1

    Sandwich ELISA standard curve of MP00372-1

    Sandwich ELISA standard curve of MP00372-1, Human MTHFR Recombinant Matched Antibody Pair - PBS only. 83372-5-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Ag14970. 83372-4-PBS was HRP conjugated as the detection antibody. Range: 0.156-10 ng/mL

  • Sample test of MP00372-1

    Sample test of MP00372-1

    K-562, Hela and HepG2 cell lysates were measured. The human MTHFR concentration of detected samples was determined to be 88.86 ng/mL (based on a 4.5 mg/mL extract load) in K-562 cell lysate, 57.89 ng/mL (based on a 3.2 mg/mL extract load) in Hela cell lysate and 55.55 ng/mL (based on a 5 mg/mL extract load) in HepG2 cell lysate.

  • Cytometric bead array standard curve of MP00372-1

    Cytometric bead array standard curve of MP00372-1

    Cytometric bead array standard curve of MP00372-1, MTHFR Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83372-5-PBS. Detection antibody: 83372-4-PBS. Standard: Ag14970. Range: 0.78-100 ng/mL.

产品信息

83372-5-PBS targets MTHFR as part of a matched antibody pair:

MP00372-1: 83372-5-PBS capture and 83372-4-PBS detection (validated in Cytometric bead array, Sandwich ELISA)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Applications Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test Application Description
Tested Reactivity human
Immunogen MTHFR fusion protein Ag14970 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name 5,10-methylenetetrahydrofolate reductase (NADPH)
Synonyms Methylenetetrahydrofolate reductase (NADPH), EC:1.5.1.53, 240348C3
Calculated Molecular Weight 656 aa, 75 kDa
GenBank Accession NumberBC053509
Gene Symbol MTHFR
Gene ID (NCBI) 4524
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP42898
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C