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NEIL1 Recombinant antibody

NEIL1 Uni-rAbTM Recombinant Antibody for IF/ICC, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

240322B7

Cat no : 83376-3-RR

Print datasheet

Synonyms

NEI L1, Endonuclease VIII-like 1, Endonuclease 8-like 1, EC:3.2.2.-, DNA-(apurinic or apyrimidinic site) lyase Neil1

验证数据展示

  • FC experiment of A549 using 83376-3-RR

    FC experiment of A549 using 83376-3-RR

    1x10^6 A549 cells were intracellularly stained with 0.25 ug Neil1 Recombinant Antibody (83376-3-RR, Clone:240322B7) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • IF Staining of HepG2 using 83376-3-RR

    IF Staining of HepG2 using 83376-3-RR

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using NEIL1 antibody (83376-3-RR, Clone: 240322B7 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

  • Affinity and Kinetic Characterization of 83376-3-RR

    Affinity and Kinetic Characterization of 83376-3-RR

    Biolayer interferometry (BLl) kinetic assays of 83376-3-RR against Human NEIL1 were performed. The affinity constant is below 1 pM.

经过测试的应用

Positive IF/ICC detected inHepG2 cells
Positive FC (Intra) detected inA549 cells

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:125-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

The immunogen of 83376-3-RR is NEIL1 Fusion Protein expressed in E. coli.

Tested Applications IF/ICC, FC (Intra), ELISA Application Description
Tested Reactivity human
Immunogen NEIL1 fusion protein Ag2793 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name nei endonuclease VIII-like 1 (E. coli)
Synonyms NEI L1, Endonuclease VIII-like 1, Endonuclease 8-like 1, EC:3.2.2.-, DNA-(apurinic or apyrimidinic site) lyase Neil1
Calculated Molecular Weight 390 aa, 44 kDa
GenBank Accession NumberBC010876
Gene Symbol NEIL1
Gene ID (NCBI) 79661
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ96FI4
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
IF protocol for NEIL1 antibody 83376-3-RRDownload protocol
FC protocol for NEIL1 antibody 83376-3-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols