XRN2 Recombinant antibody
XRN2 Recombinant Antibody for WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
Human
Applications
WB, ELISA
Conjugate
Unconjugated
CloneNo.
240065G4
验证数据展示
经过测试的应用
Positive WB detected in | MCF-7 cells, Jurkat cells, HeLa cells |
Planning an IHC experiment? We recommend our IHCeasy XRN2 Ready-To-Use IHC Kit. XRN2 primary antibody included.
推荐稀释比
Application | Dilution |
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Western Blot (WB) | WB : 1:5000-1:50000 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
The immunogen of 83378-1-RR is XRN2 Fusion Protein expressed in E. coli.
Tested Applications | WB, ELISA Application Description |
Tested Reactivity | Human |
Immunogen | XRN2 fusion protein Ag30781 种属同源性预测 |
Host / Isotype | Rabbit / IgG |
Class | Recombinant |
Type | Antibody |
Full Name | 5'-3' exoribonuclease 2 |
Synonyms | 5 3 exoribonuclease 2, 5' 3' exoribonuclease 2, DHM1 like protein, DHP protein, XRN2 |
Calculated Molecular Weight | 104 kDa |
Observed Molecular Weight | 109 kDa |
GenBank Accession Number | BC006417 |
Gene Symbol | XRN2 |
Gene ID (NCBI) | 22803 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Protein A purfication |
UNIPROT ID | Q9H0D6 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
XRN2 is one exonuclease that degrades the Pol II associated product of poly(A) site cleavage, which is crucial for Pol II termination. During transcription termination, XRN2 cleavages at the polyadenylation site liberates a 5' fragment which is subsequently processed to form the mature mRNA and a 3' fragment which remains attached to the elongating polymerase. The processive degradation of this 3' fragment by this protein may promote termination of transcription.
实验方案
Product Specific Protocols | |
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WB protocol for XRN2 antibody 83378-1-RR | Download protocol |
FC protocol for XRN2 antibody 83378-1-RR | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |