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验证数据展示

  • WB analysis using 83401-4-RR

    WB analysis using 83401-4-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 83401-4-RR (TSSK4 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse testis using 83401-4-RR

    IHC staining of mouse testis using 83401-4-RR

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 83401-4-RR (TSSK4 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of mouse testis using 83401-4-RR

    IF Staining of mouse testis using 83401-4-RR

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse testis tissue using TSSK4 antibody (83401-4-RR, Clone: 240373F10 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Affinity and Kinetic Characterization of 83401-4-RR

    Affinity and Kinetic Characterization of 83401-4-RR

    Biolayer interferometry (BLl) kinetic assays of 83401-4-RR against Human TSSK4 were performed. The affinity constant is 5.41 nM.

TSSK4 Recombinant antibody

TSSK4 Uni-rAbTM Recombinant Antibody for WB, IHC, IF-P, ELISA
Cat No. 83401-4-RR

产品说明书

CloneNo. 240373F10

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF-P, ELISA

别名

testis serine kinase 4, STK22E, Serine/threonine-protein kinase 22E, EC:2.7.11.1, C14orf20

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inA549 cells, HT-29 cells, COLO 320 cells, mouse testis tissue, mouse colon tissue, rat testis tissue
Positive IHC detected inmouse testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inmouse testis tissue

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)-PIF-P : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

83401-4-RR targets TSSK4 in WB, IHC, IF-P, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF-P, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 TSSK4 fusion protein Ag16205 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 testis-specific serine kinase 4
别名 testis serine kinase 4, STK22E, Serine/threonine-protein kinase 22E, EC:2.7.11.1, C14orf20
计算分子量 338 aa, 37 kDa
观测分子量 38-45 kDa
GenBank蛋白编号BC111088
基因名称 TSSK4
Gene ID (NCBI) 283629
RRIDAB_3671052
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ6SA08
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

TSSK4 (Testis-specific serine/threonine-protein kinase 4), testis-specific serine/threonine protein kinase 4, is a member of the testis-specific serine/threonine protein kinase family. It has autophosphorylation activity and self-binding properties; it can interact with and phosphorylate other proteins, e.g., it can bind to and phosphorylate Odf2. It's reported that TSSK4 plays an important role in spermatogenesis, TSSK4 knockout male mice develop reduced sperm motility and reduced fertility. In addition, TSSK4 regulates lung fibrosis by promoting the loss of alveolar epithelial type II cells through the HSP90-AKT signaling pathway.

实验方案

Product Specific Protocols
WB protocol for TSSK4 antibody 83401-4-RRDownload protocol
IHC protocol for TSSK4 antibody 83401-4-RRDownload protocol
IF protocol for TSSK4 antibody 83401-4-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols