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验证数据展示

  • WB analysis using 83418-5-RR

    WB analysis using 83418-5-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 83418-5-RR (KIAA1715 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • IF Staining of U-251 using 83418-5-RR

    IF Staining of U-251 using 83418-5-RR

    Immunofluorescent analysis of (4% PFA) fixed U-251 cells using KIAA1715 antibody (83418-5-RR, Clone: 240211B9 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • IHC staining of mouse cerebellum using 83418-5-RR

    IHC staining of mouse cerebellum using 83418-5-RR

    Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 83418-5-RR (KIAA1715 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

KIAA1715 Recombinant antibody

KIAA1715 Uni-rAbTM Recombinant Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 83418-5-RR

产品说明书

CloneNo. 240211B9

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, IF/ICC, ELISA

别名

LNP, Ul, Protein lunapark, LNPK, ER junction formation factor lunapark

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHEK-293 cells, K-562 cells, HepG2 cells, U2OS cells, mouse testis tissue, mouse colon tissue, mouse kidney tissue
Positive IHC detected inmouse cerebellum tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inU-251 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:2000-1:10000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

83418-5-RR targets KIAA1715 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
经测试反应性 human, mouse
免疫原 KIAA1715 fusion protein Ag34669 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 KIAA1715
别名 LNP, Ul, Protein lunapark, LNPK, ER junction formation factor lunapark
观测分子量53-55 kDa
GenBank蛋白编号BC105132
基因名称 KIAA1715
Gene ID (NCBI) 80856
RRIDAB_3671067
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purfication
UNIPROT IDQ9C0E8
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

KIAA1715, also known as LNPK, is a member of the conserved Lunapark protein family. KIAA1715 is an endoplasmic reticulum (ER) membrane protein consisting of an N-terminal domain important for N-myristoylation and ER targeting, two transmembrane domains and a unique zinc finger domain. It has 4 isoforms produced by alternative splicing and acts mainly as a stabilizer of negative membrane curvature (PMID: 27619977).

实验方案

Product Specific Protocols
WB protocol for KIAA1715 antibody 83418-5-RRDownload protocol
IHC protocol for KIAA1715 antibody 83418-5-RRDownload protocol
IF protocol for KIAA1715 antibody 83418-5-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols