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验证数据展示

  • WB analysis using 83422-1-RR

    WB analysis using 83422-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 83422-1-RR (cystatin c antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse kidney using 83422-1-RR

    IHC staining of mouse kidney using 83422-1-RR

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 83422-1-RR (cystatin c antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse kidney using 83422-1-RR

    IHC staining of mouse kidney using 83422-1-RR

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 83422-1-RR (cystatin c antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Affinity and Kinetic Characterization of 83422-1-RR

    Affinity and Kinetic Characterization of 83422-1-RR

    Biolayer interferometry (BLl) kinetic assays of 83422-1-RR against Mouse Cystatin C were performed. The affinity constant is 20.3 nM.

Cystatin C Recombinant antibody

Cystatin C Uni-rAbTM Recombinant Antibody for WB, IHC, ELISA
Cat No. 83422-1-RR
CloneNo. 240202A2

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Synonyms

Cst3, CysC, 240202A2

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size: 

-/ -

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经过测试的应用

Positive WB detected inRAW 264.7 cells, mouse brain tissue
Positive IHC detected inmouse kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

83422-1-RR targets Cystatin C in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications WB, IHC, ELISA Application Description
Tested Reactivity human, mouse
Immunogen Fusion Protein 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name cystatin C
Synonyms Cst3, CysC, 240202A2
Calculated Molecular Weight15KD
Observed Molecular Weight 13-16 kDa
GenBank Accession NumberNM_009976.4
Gene Symbol Cystatin C
Gene ID (NCBI) 13010
RRIDAB_3671071
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDP21460
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Cystatin C is a 13-kDa inhibitor of cysteine proteinases which is secreted by all cell types and is completely cleared from the organism through glomerular filtration, shown to be an early and sensitive biomarker of renal dysfunction. It is also used as an emerging biomarker in cardiovascular disease. Cystatin C is involved in a variety of inflammatory reactions. The concentration of serum cystatin C has also been shown to be unaltered in certain inflammatory conditions or other disorders of metabolism. The plasma level of serum cystatin C can be expressed as its level of generation from cells and diet and its subsequent elimination through the gut, liver, and kidney.

实验方案

Product Specific Protocols
WB protocol for Cystatin C antibody 83422-1-RRDownload protocol
IHC protocol for Cystatin C antibody 83422-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols