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TREM2 Recombinant antibody, PBS Only (Capture/Detector)

TREM2 Uni-rAbTM Recombinant Antibody for Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Conjugate

Unconjugated

CloneNo.

240384G3

Cat no : 83438-5-PBS

Print datasheet

Synonyms

Trem2c, Trem2b, Trem2a, TREM-2, TREM 2

验证数据展示

  • Sandwich ELISA standard curve of MP00440-4

    Sandwich ELISA standard curve of MP00440-4

    Sandwich ELISA standard curve of MP00440-4, Human TREM2 Monoclonal Matched Antibody Pair - PBS only. 83438-5-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Eg0747. 83438-7-PBS was HRP conjugated as the detection antibody. Range: 23.4-1500 pg/mL.

  • Sample test of MP00440-4

    Sample test of MP00440-4

    THP-1 cells (3 x 10^6 cells/mL) were cultured in RPMI with 10% fetal bovine serum, 50 μM β-mercaptoethanol, 2 mM L-glutamine, 100 U/mL penicillin, and 100 μg/mL streptomycin sulfate. Aliquots of the cell culture supernatants were removed and assayed for levels of human TREM2 and measured 1,626.6 pg/mL.

  • Sample test of MP00440-4

    Sample test of MP00440-4

    The mean human TREM2 concentration was determined to be 5,362.2 pg/mL in THP-1 cell extract based on a 3.0 mg/mL extract load.

  • Sample test of MP00440-4

    Sample test of MP00440-4

    Serum of sixteen individual healthy human donors were measured. The human TREM2 concentration of detected samples was determined to be 2,096.2 pg/mL with a range of 661.9-6,705.0 pg/mL.

  • Cytometric bead array standard curve of MP00440-2

    Cytometric bead array standard curve of MP00440-2

    Cytometric bead array standard curve of MP00440-2, TREM2 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83438-3-PBS. Detection antibody: 83438-5-PBS. Standard: Eg0747. Range: 0.313-40 ng/mL.

  • Cytometric bead array standard curve of MP00440-3

    Cytometric bead array standard curve of MP00440-3

    Cytometric bead array standard curve of MP00440-3, TREM2 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83438-4-PBS. Detection antibody: 83438-5-PBS. Standard: Eg0747. Range: 0.313-40 ng/mL.

Planning an IHC experiment? We recommend our IHCeasy TREM2 Ready-To-Use IHC Kit. TREM2 primary antibody included.

产品信息

83438-5-PBS targets TREM2 as part of a matched antibody pair:

MP00440-2: 83438-3-PBS capture and 83438-5-PBS detection (validated in Cytometric bead array)

MP00440-3: 83438-4-PBS capture and 83438-5-PBS detection (validated in Cytometric bead array)

MP00440-4: 83438-5-PBS capture and 83438-7-PBS detection (validated in Sandwich ELISA)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Applications Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test Application Description
Tested Reactivity human
Immunogen Fusion Protein 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name triggering receptor expressed on myeloid cells 2
Synonyms Trem2c, Trem2b, Trem2a, TREM-2, TREM 2
Calculated Molecular Weight 222 aa, 25 kDa
GenBank Accession NumberBC032362
Gene Symbol TREM2
Gene ID (NCBI) 54209
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ9NZC2
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C