• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

验证数据展示

  • WB analysis using 83452-4-RR

    WB analysis using 83452-4-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 83452-4-RR (GNB3 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • FC experiment of HepG2 using 83452-4-RR

    FC experiment of HepG2 using 83452-4-RR

    1x10^6 HepG2 cells were intracellularly stained with 0.25 ug GNB3 Recombinant antibody (83452-4-RR, Clone:240281D1) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • Affinity and Kinetic Characterization of 83452-4-RR

    Affinity and Kinetic Characterization of 83452-4-RR

    Biolayer interferometry (BLl) kinetic assays of 83452-4-RR against Human GNB3 were performed. The affinity constant is below 1 pM.

GNB3 Recombinant antibody

GNB3 Uni-rAbTM Recombinant Antibody for WB, FC (Intra), ELISA
Cat No. 83452-4-RR

产品说明书

CloneNo. 240281D1

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, FC (Intra), ELISA

别名

Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-3, 240281D1

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

点击咨询售前客服

点击咨询售后客服

经过测试的应用

Positive WB detected inMCF-7 cells, L02 cells, HepG2 cells, C6 cells, mouse brain tissue, mouse cerebellum tissue, rat brain tissue
Positive FC (Intra) detected inHepG2 cells, HeLa cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

83452-4-RR targets GNB3 in WB, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, FC (Intra), ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 GNB3 fusion protein Ag0127 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 guanine nucleotide binding protein (G protein), beta polypeptide 3
别名 Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-3, 240281D1
计算分子量 37 kDa
观测分子量 35-37 kDa
GenBank蛋白编号BC000115
基因名称 GNB3
Gene ID (NCBI) 2784
RRIDAB_3671089
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purfication
UNIPROT IDP16520
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Guanine nucleotide-binding proteins (g proteins) are involved as a modulator or transducer in various transmembrane signaling systems, by integrating signals between receptors and effector proteins. G proteins are composed of an alpha, a beta, and a gamma subunit. This gene encodes a 34 kd beta subunit, being expressed in all tissues. Beta subunits are important regulators of alpha subunits, as well as of certain signal transduction receptors and effectors.

实验方案

Product Specific Protocols
WB protocol for GNB3 antibody 83452-4-RRDownload protocol
FC protocol for GNB3 antibody 83452-4-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols