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DFNA5 Recombinant antibody

DFNA5 Uni-rAbTM Recombinant Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

240394D2

Cat no : 83454-6-RR

Print datasheet

Synonyms

Gasdermin-E, Gasdermin E, DFNA5/GSDME, GSDME, DFNA 5

验证数据展示

  • WB analysis using 83454-6-RR

    WB analysis using 83454-6-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 83454-6-RR (DFNA5 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Affinity and Kinetic Characterization of 83454-6-RR

    Affinity and Kinetic Characterization of 83454-6-RR

    Biolayer interferometry (BLl) kinetic assays of 83454-6-RR against Human DFNA5 were performed. The affinity constant is below 1 pM.

经过测试的应用

Positive WB detected inHeLa cells, SH-SY5Y cells, mouse brain tissue, rat brain tissue
Planning an IHC experiment? We recommend our IHCeasy GSDME/DFNA5 Ready-To-Use IHC Kit. GSDME/DFNA5 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

The immunogen of 83454-6-RR is DFNA5 Fusion Protein expressed in E. coli.

Tested Applications WB, ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen DFNA5 fusion protein Ag3746 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name deafness, autosomal dominant 5
Synonyms Gasdermin-E, Gasdermin E, DFNA5/GSDME, GSDME, DFNA 5
Calculated Molecular Weight 496 aa, 55 kDa
Observed Molecular Weight55 kDa
GenBank Accession NumberBC019689
Gene Symbol DFNA5
Gene ID (NCBI) 1687
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDO60443
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

DFNA5 (deafness, autosomal dominant 5), also known as GSDME or ICERE-1, is a 496 amino acid protein that is expressed in cochlea tissue, as well as in placenta, brain, heart, liver, lung and pancreas. Defects in the gene encoding DFNA5 are the cause of non-syndromic sensorineural deafness autosomal dominant type 5 (DFNA5), a form of sensorineural hearing loss that results from damage to one of various structures that receive sound information in the brain. GSDME produced two GSDME fragments with MW of 35 kDa and 25 kDa.

实验方案

Product Specific Protocols
WB protocol for DFNA5 antibody 83454-6-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols