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验证数据展示

  • WB analysis using 83463-8-RR

    WB analysis using 83463-8-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 83463-8-RR (TRMT2A antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse liver using 83463-8-RR

    IHC staining of mouse liver using 83463-8-RR

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 83463-8-RR (TRMT2A antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • FC experiment of U-251 using 83463-8-RR

    FC experiment of U-251 using 83463-8-RR

    1x10^6 U-251 cells were intracellularly stained with 0.25 ug TRMT2A Recombinant antibody (83463-8-RR, Clone:240444C2) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • Affinity and Kinetic Characterization of 83463-8-RR

    Affinity and Kinetic Characterization of 83463-8-RR

    Biolayer interferometry (BLl) kinetic assays of 83463-8-RR against Human TRMT2A were performed. The affinity constant is 1.86 nM.

TRMT2A Recombinant antibody

TRMT2A Uni-rAbTM Recombinant Antibody for WB, IHC, FC (Intra), ELISA
Cat No. 83463-8-RR

产品说明书

CloneNo. 240444C2

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, FC (Intra), ELISA

别名

tRNA (uracil 5 ) methyltransferase homolog A, mRNA, HTF9C, EC:2.1.1.35, EC:2.1.1.-

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inK-562 cells, A431 cells, MCF-7 cells, MOLT-4 cells, HepG2 cells
Positive IHC detected inmouse liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive FC (Intra) detected inU-251 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:200-1:800
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

83463-8-RR targets TRMT2A in WB, IHC, FC (Intra), ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, FC (Intra), ELISA Application Description
经测试反应性 human, mouse
免疫原 TRMT2A fusion protein Ag9112 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 TRM2 tRNA methyltransferase 2 homolog A (S. cerevisiae)
别名 tRNA (uracil 5 ) methyltransferase homolog A, mRNA, HTF9C, EC:2.1.1.35, EC:2.1.1.-
计算分子量 625 aa, 69 kDa
观测分子量 42-44 kDa, 68-75 kDa
GenBank蛋白编号BC013352
基因名称 TRMT2A
Gene ID (NCBI) 27037
RRIDAB_3671094
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purfication
UNIPROT IDQ8IZ69
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

tRNA methyltransferase 2 homolog A (TRMT2A), also known as HTF9C is a novel cell cycle regulated protein, as associated with aggressive clinical course. HTF9C harbors a putative RNA recognition motif (RRM) in the N-terminus and the conserved uracil-C5-methyltransferase domain of the TrmA family in the C-terminus. HTF9C antibody can detect two bands about 68 kDa and 40 kDa (PMID: 20307320).

实验方案

Product Specific Protocols
WB protocol for TRMT2A antibody 83463-8-RRDownload protocol
IHC protocol for TRMT2A antibody 83463-8-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols