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验证数据展示

  • FC experiment of MCF-7 using 83467-2-RR (same clone as 83467-2-PBS)

    FC experiment of MCF-7 using 83467-2-RR (same clone as 83467-2-PBS)

    1x10^6 MCF-7 cells were intracellularly stained with 0.25 ug Tff1 Recombinant Antibody (83467-2-RR, Clone:240424F5) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 83467-2-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP00469-1

    Cytometric bead array standard curve of MP00469-1

    Cytometric bead array standard curve of MP00469-1, TFF1 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83467-2-PBS. Detection antibody: 83467-1-PBS. Standard: Ag4541. Range: 0.313-40 ng/mL.

  • Affinity and Kinetic Characterization of 83467-2-RR

    Affinity and Kinetic Characterization of 83467-2-RR

    Biolayer interferometry (BLl) kinetic assays of 83467-2-RR against Human TFF1 were performed. The affinity constant is 0.296 nM.

TFF1 Recombinant antibody, PBS Only (Capture)

TFF1 Uni-rAbTM Recombinant Antibody for FC (Intra), Cytometric bead array, Indirect ELISA
Cat No. 83467-2-PBS

产品说明书

CloneNo. 240424F5

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

FC (Intra), Cytometric bead array, Indirect ELISA

别名

Polypeptide P1.A, PNR-2, pNR 2, HP1.A, Breast cancer estrogen-inducible protein

缓冲液配方:  PBS Only
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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Planning an IHC experiment? We recommend our IHCeasy TFF1 Ready-To-Use IHC Kit. TFF1 primary antibody included.

产品信息

83467-2-PBS targets TFF1 as part of a matched antibody pair:

MP00469-1: 83467-2-PBS capture and 83467-1-PBS detection (validated in Cytometric bead array)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

经测试应用 FC (Intra), Cytometric bead array, Indirect ELISA Application Description
经测试反应性 human
免疫原 TFF1 fusion protein Ag4541 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 trefoil factor 1
别名 Polypeptide P1.A, PNR-2, pNR 2, HP1.A, Breast cancer estrogen-inducible protein
计算分子量 84 aa, 9 kDa
GenBank蛋白编号BC032811
基因名称 TFF1
Gene ID (NCBI) 7031
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP04155
储存缓冲液 PBS Only
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

TFF1 (Trefoil factor 1, formerly known as pS2) is a small-secreted protein that belongs to the family of trefoil peptides, and characterized by the presence of one to six cysteine-rich P domains. TFF1 expression is frequently lost in gastric carcinoma, probably through mechanism of DNA methylation, and it is therefore considered as a tumor suppressor gene. The function of TFF1 was first controversially considered as an oncogene in breast cancer.