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验证数据展示

  • WB analysis using 83489-1-RR (same clone as 83489-1-PBS)

    WB analysis using 83489-1-RR (same clone as 83489-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 83489-1-RR (ARID4A antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83489-1-PBS in a different storage buffer formulation.

  • IF Staining of MCF-7 using 83489-1-RR (same clone as 83489-1-PBS)

    IF Staining of MCF-7 using 83489-1-RR (same clone as 83489-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using ARID4A antibody (83489-1-RR, Clone: 240436A4 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 83489-1-PBS in a different storage buffer formulation.

  • FC experiment of U2OS using 83489-1-RR (same clone as 83489-1-PBS)

    FC experiment of U2OS using 83489-1-RR (same clone as 83489-1-PBS)

    1x10^6 U2OS cells were intracellularly stained with 0.25 ug ARID4A Recombinant antibody (83489-1-RR, Clone:240436A4) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 83489-1-PBS in a different storage buffer formulation.

  • Sandwich ELISA standard curve of MP00489-3

    Sandwich ELISA standard curve of MP00489-3

    Sandwich ELISA standard curve of MP00489-3, Human ARID4A Recombinant Matched Antibody Pair - PBS only. 83489-1-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Ag33114. 83489-2-PBS was HRP conjugated as the detection antibody. Range: 78.1-5000 pg/mL.

  • Sample test of MP00489-3

    Sample test of MP00489-3

    The mean ARID4A concentration was determined to be 9.3 ng/mL in DU 145 cell extract based on a 3.2 mg/mL extract load, 18.1 ng/mL in MCF-7 cell extract based on a 3.5 mg/mL extract load.

  • IHC staining of mouse brain using 83489-1-RR (same clone as 83489-1-PBS)

    IHC staining of mouse brain using 83489-1-RR (same clone as 83489-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 83489-1-RR (ARID4A antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83489-1-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP00489-1

    Cytometric bead array standard curve of MP00489-1

    Cytometric bead array standard curve of MP00489-1, ARID4A Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83489-1-PBS. Detection antibody: 83489-4-PBS. Standard: Ag33114. Range: 0.313-40 ng/mL.

  • Affinity and Kinetic Characterization of 83489-1-RR

    Affinity and Kinetic Characterization of 83489-1-RR

    Biolayer interferometry (BLl) kinetic assays of 83489-1-RR against Human ARID4A were performed. The affinity constant is below 1 pM.

ARID4A Recombinant antibody, PBS Only (Capture)

ARID4A Uni-rAbTM Recombinant Antibody for WB, IHC, IF/ICC, FC (Intra), Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test
Cat No. 83489-1-PBS

产品说明书

CloneNo. 240436A4

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, IF/ICC, FC (Intra), Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

别名

RBP1, RBP 1, RBBP1, RBBP 1, ARID 4A

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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产品信息

83489-1-PBS targets ARID4A as part of a matched antibody pair:

MP00489-1: 83489-1-PBS capture and 83489-4-PBS detection (validated in Cytometric bead array)

MP00489-3: 83489-1-PBS capture and 83489-2-PBS detection (validated in Sandwich ELISA)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

经测试应用 WB, IHC, IF/ICC, FC (Intra), Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test Application Description
经测试反应性 human
免疫原 ARID4A fusion protein Ag33114 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 AT rich interactive domain 4A (RBP1-like)
别名 RBP1, RBP 1, RBBP1, RBBP 1, ARID 4A
计算分子量143 kDa
观测分子量205 kDa
GenBank蛋白编号NM_002892.3
基因名称 ARID4A
Gene ID (NCBI) 5926
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP29374
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

ARID4A (AT-rich interaction domain 4A, also known as retinoblastoma-binding protein 1, RBBP1/RBP1) is a retinoblastoma (RB)-binding protein. ARID1A is an important subunit of the switching defective/sucrose nonfermenting (SWI/SNF) chromatin remodeling complex.