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验证数据展示

  • WB analysis using 83489-3-RR

    WB analysis using 83489-3-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 83489-3-RR (ARID4A antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Affinity and Kinetic Characterization of 83489-3-RR

    Affinity and Kinetic Characterization of 83489-3-RR

    Biolayer interferometry (BLl) kinetic assays of 83489-3-RR against Human ARID4A were performed. The affinity constant is below 1 pM.

ARID4A Recombinant antibody

ARID4A Uni-rAbTM Recombinant Antibody for WB, ELISA
Cat No. 83489-3-RR

产品说明书

CloneNo. 240436C9

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, ELISA

别名

RBP1, RBP 1, RBBP1, RBBP 1, ARID 4A

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHeLa cells, MCF-7 cells, LNCaP cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

83489-3-RR targets ARID4A in WB, ELISA applications and shows reactivity with human samples.

经测试应用 WB, ELISA Application Description
经测试反应性 human
免疫原 ARID4A fusion protein Ag33114 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 AT rich interactive domain 4A (RBP1-like)
别名 RBP1, RBP 1, RBBP1, RBBP 1, ARID 4A
计算分子量143 kDa
观测分子量205 kDa
GenBank蛋白编号NM_002892.3
基因名称 ARID4A
Gene ID (NCBI) 5926
RRIDAB_3671116
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP29374
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

ARID4A (AT-rich interaction domain 4A, also known as retinoblastoma-binding protein 1, RBBP1/RBP1) is a retinoblastoma (RB)-binding protein. ARID1A is an important subunit of the switching defective/sucrose nonfermenting (SWI/SNF) chromatin remodeling complex.

实验方案

Product Specific Protocols
WB protocol for ARID4A antibody 83489-3-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols