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验证数据展示

  • WB analysis using 83497-1-RR (same clone as 83497-1-PBS)

    WB analysis using 83497-1-RR (same clone as 83497-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 83497-1-RR (HARS antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83497-1-PBS in a different storage buffer formulation.

  • IHC staining of human intrahepatic cholangiocarcinoma using 83497-1-RR (same clone as 83497-1-PBS)

    IHC staining of human intrahepatic cholangiocarcinoma using 83497-1-RR (same clone as 83497-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 83497-1-RR (HARS antibody) at dilution of 1:250 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83497-1-PBS in a different storage buffer formulation.

  • IHC staining of human intrahepatic cholangiocarcinoma using 83497-1-RR (same clone as 83497-1-PBS)

    IHC staining of human intrahepatic cholangiocarcinoma using 83497-1-RR (same clone as 83497-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 83497-1-RR (HARS antibody) at dilution of 1:250 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83497-1-PBS in a different storage buffer formulation.

  • Sandwich ELISA standard curve of MP00486-1

    Sandwich ELISA standard curve of MP00486-1

    Sandwich ELISA standard curve of MP00486-1, Human HARS Recombinant Matched Antibody Pair - PBS only. 83497-1-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Ag9333. 83497-2-PBS was HRP conjugated as the detection antibody. Range: 39.1-2500 pg/mL

  • Sample test of MP00486-1

    Sample test of MP00486-1

    MCF-7, Hela and HEK-293 cell lysates were measured. The human HARS concentration of detected samples was determined to be 579.62 ng/mL (based on a 4.3 mg/mL extract load) in MCF-7 cell lysate, 830.79 ng/mL (based on a 3.6 mg/mL extract load) in Hela cell lysate and 698.03 ng/mL (based on a 3.4 mg/mL extract load) in HEK-293 cell lysate.

  • IF Staining of MCF-7 using 83497-1-RR (same clone as 83497-1-PBS)

    IF Staining of MCF-7 using 83497-1-RR (same clone as 83497-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using HARS antibody (83497-1-RR, Clone: 240495C1 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 83497-1-PBS in a different storage buffer formulation.

  • IF Staining of MCF-7 using 83497-1-RR (same clone as 83497-1-PBS)

    IF Staining of MCF-7 using 83497-1-RR (same clone as 83497-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using HARS antibody (83497-1-RR, Clone: 240495C1 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 83497-1-PBS in a different storage buffer formulation.

  • FC experiment of MCF-7 using 83497-1-RR (same clone as 83497-1-PBS)

    FC experiment of MCF-7 using 83497-1-RR (same clone as 83497-1-PBS)

    1x10^6 MCF-7 cells were intracellularly stained with 0.25 ug HARS Recombinant antibody (83497-1-RR, Clone:240495C1) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 83497-1-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP00486-1

    Cytometric bead array standard curve of MP00486-1

    Cytometric bead array standard curve of MP00486-1, HARS Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83497-1-PBS. Detection antibody: 83497-2-PBS. Standard: Ag9333. Range: 0.78-100 ng/mL.

  • Affinity and Kinetic Characterization of 83497-1-RR

    Affinity and Kinetic Characterization of 83497-1-RR

    Biolayer interferometry (BLl) kinetic assays of 83497-1-RR against Human HARS were performed. The affinity constant is 0.382 nM.

HARS Recombinant antibody, PBS Only (Capture)

HARS Uni-rAbTM Recombinant Antibody for WB, IHC, IF/ICC, FC (Intra), Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test
Cat No. 83497-1-PBS

产品说明书

CloneNo. 240495C1

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, IF/ICC, FC (Intra), Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

别名

Histidyl-tRNA synthetase, Histidine--tRNA ligase, cytoplasmic, HisRS, HARS1, EC:6.1.1.21

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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产品信息

83497-1-PBS targets HARS as part of a matched antibody pair:

MP00486-1: 83497-1-PBS capture and 83497-2-PBS detection (validated in Cytometric bead array, Sandwich ELISA)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

经测试应用 WB, IHC, IF/ICC, FC (Intra), Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test Application Description
经测试反应性 human
免疫原 HARS fusion protein Ag9333 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 histidyl-tRNA synthetase
别名 Histidyl-tRNA synthetase, Histidine--tRNA ligase, cytoplasmic, HisRS, HARS1, EC:6.1.1.21
计算分子量 509 aa, 57 kDa
观测分子量52 kDa
GenBank蛋白编号BC011807
基因名称 HARS
Gene ID (NCBI) 3035
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP12081
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

HARS is a cytoplasmic enzyme that belongs to the class II family of aminoacyl-tRNA synthetases. The enzyme is responsible for the synthesis of histidyl-transfer RNA, which is essential for the incorporation of histidine into proteins. HARS is located in a head-to-head orientation with HARSL on chromosome five, where the homologous genes share a bidirectional promoter. The gene product is a frequent target of autoantibodies in the human autoimmune disease polymyositis/dermatomyositis.