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Integrin beta-5 Recombinant antibody, PBS Only (Capture/Detector)

Integrin beta-5 Uni-rAbTM Recombinant Antibody for WB, IHC, Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Conjugate

Unconjugated

CloneNo.

240401G11

Cat no : 83514-3-PBS

Print datasheet

Synonyms

ITGB5, integrin, beta 5, Integrin beta 5, 240401G11

验证数据展示

  • WB analysis using 83514-3-RR (same clone as 83514-3-PBS)

    WB analysis using 83514-3-RR (same clone as 83514-3-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 83514-3-RR (Integrin beta-5 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83514-3-PBS in a different storage buffer formulation.

  • Sandwich ELISA standard curve of MP00512-4

    Sandwich ELISA standard curve of MP00512-4

    Sandwich ELISA standard curve of MP00512-4, Integrin beta-5 Recombinant Matched Antibody Pair - PBS only. 83514-3-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Ag29783.83514-1-PBS was HRP conjugated as the detection antibody. Range: 9.766-625 pg/mL.

  • Sample test of MP00512-4

    Sample test of MP00512-4

    The mean Integrin beta-5 concentration was determined to be 275.71 pg/mL in Hela cell extract based on a 2.8 mg/mL extract load and 190.06 pg/mL in MCF-7 cell extract based on a 3.1 mg/mL extract load.

  • IHC staining of human ovary cancer using 83514-3-RR (same clone as 83514-3-PBS)

    IHC staining of human ovary cancer using 83514-3-RR (same clone as 83514-3-PBS)

    Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 83514-3-RR (Integrin beta-5 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83514-3-PBS in a different storage buffer formulation.

  • IHC staining of human ovary cancer using 83514-3-RR (same clone as 83514-3-PBS)

    IHC staining of human ovary cancer using 83514-3-RR (same clone as 83514-3-PBS)

    Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 83514-3-RR (Integrin beta-5 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83514-3-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP00512-1

    Cytometric bead array standard curve of MP00512-1

    Cytometric bead array standard curve of MP00512-1, Integrin beta-5 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83514-2-PBS. Detection antibody: 83514-3-PBS. Standard: Ag29783. Range: 0.313-40 ng/mL.

  • Cytometric bead array standard curve of MP00512-2

    Cytometric bead array standard curve of MP00512-2

    Cytometric bead array standard curve of MP00512-2, Integrin beta-5 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83514-5-PBS. Detection antibody: 83514-3-PBS. Standard: Ag29783. Range: 0.313-40 ng/mL.

  • Affinity and Kinetic Characterization of 83514-3-RR

    Affinity and Kinetic Characterization of 83514-3-RR

    Biolayer interferometry (BLl) kinetic assays of 83514-3-RR against Human Integrin beta-5 were performed. The affinity constant is 0.955 nM.

产品信息

83514-3-PBS targets Integrin beta-5 as part of a matched antibody pair:

MP00512-1: 83514-2-PBS capture and 83514-3-PBS detection (validated in Cytometric bead array)

MP00512-2: 83514-5-PBS capture and 83514-3-PBS detection (validated in Cytometric bead array)

MP00512-4: 83514-3-PBS capture and 83514-1-PBS detection (validated in Sandwich ELISA)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Applications WB, IHC, Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test Application Description
Tested Reactivity human
Immunogen Integrin beta-5 fusion protein Ag29783 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name integrin, beta 5
Synonyms ITGB5, integrin, beta 5, Integrin beta 5, 240401G11
Calculated Molecular Weight 88 kDa
Observed Molecular Weight88-100 kDa
GenBank Accession NumberBC006541
Gene Symbol Integrin beta 5
Gene ID (NCBI) 3693
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP18084
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

ITGB5, also known as Integrin beta-5. It is expected to be located in the plasma membrane and mitochondria, which is i ubiquitinated in placenta and gall bladder. ITGB5 is a member of integrins and was proved to be associated with pathological processes in several tumors (PMID: 34966851). It is reported that TGB5 is highly expressed in hepatocellular carcinoma (HCC), and miR-185 regulates the expression of β- catenin through the ITGB5-dependent manner and affects the proliferation and migration of HCC cells (PMID: 35223869). The molecular weight of ITGB5 is 88 kDa, and the glycosylation modification also exists.