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验证数据展示

  • WB analysis using 83517-3-RR

    WB analysis using 83517-3-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 83517-3-RR (RPS11 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse testis using 83517-3-RR

    IHC staining of mouse testis using 83517-3-RR

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 83517-3-RR (RPS11 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse testis using 83517-3-RR

    IHC staining of mouse testis using 83517-3-RR

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 83517-3-RR (RPS11 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human stomach cancer using 83517-3-RR

    IHC staining of human stomach cancer using 83517-3-RR

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 83517-3-RR (RPS11 antibody) at dilution of 1:4000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer(pH9).

  • IF Staining of HepG2 using 83517-3-RR

    IF Staining of HepG2 using 83517-3-RR

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using RPS11 antibody (83517-3-RR, Clone: 240327D11 ) at dilution of 1:250 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-Phalloidin (red).

  • FC experiment of MCF-7 using 83517-3-RR

    FC experiment of MCF-7 using 83517-3-RR

    1x10^6 MCF-7 cells were intracellularly stained with 0.25 ug RPS11 Recombinant antibody (83517-3-RR, Clone:240327D11) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • Affinity and Kinetic Characterization of 83517-3-RR

    Affinity and Kinetic Characterization of 83517-3-RR

    Biolayer interferometry (BLl) kinetic assays of 83517-3-RR against Human RPS11 were performed. The affinity constant is 5.05 nM.

RPS11 Recombinant antibody

RPS11 Recombinant Antibody for WB, IHC, IF/ICC, FC (Intra), ELISA
Cat No. 83517-3-RR

产品说明书

CloneNo. 240327D11

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, FC (Intra), ELISA

别名

Small ribosomal subunit protein uS17, ribosomal protein S11, 40S ribosomal protein S11, 240327D11

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
CoraLite® Plus 488
规格: 

-/ -

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经过测试的应用

Positive WB detected inROS1728 cells, mouse testis tissue, rat testis tissue
Positive IHC detected inmouse testis tissue, human stomach cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells
Positive FC (Intra) detected inMCF-7 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:200-1:800
Immunofluorescence (IF)/ICCIF/ICC : 1:125-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

83517-3-RR targets RPS11 in WB, IHC, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, FC (Intra), ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 RPS11 fusion protein Ag34848 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 ribosomal protein S11
别名 Small ribosomal subunit protein uS17, ribosomal protein S11, 40S ribosomal protein S11, 240327D11
计算分子量 158 aa, 18 kDa
观测分子量18 kDa
GenBank蛋白编号BC007945
基因名称 RPS11
Gene ID (NCBI) 6205
RRIDAB_3671142
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purfication
UNIPROT IDP62280
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

RPS11 is a ribosomal protein involved in ribosome biogenesis. It is a component of the small ribosomal subunit. The ribosome is a large ribonucleoprotein complex responsible for the synthesis of proteins in the cell.

实验方案

Product Specific Protocols
WB protocol for RPS11 antibody 83517-3-RRDownload protocol
IHC protocol for RPS11 antibody 83517-3-RRDownload protocol
IF protocol for RPS11 antibody 83517-3-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols