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Conjugate
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CD5 Recombinant antibody

CD5 Uni-rAbTM Recombinant Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

240428G2

Cat no : 83543-7-RR

Print datasheet

Synonyms

T1, Lymphocyte antigen T1/Leu-1, Lymphocyte antigen T1/Leu 1, LEU1, CD5 molecule

验证数据展示

  • WB analysis of Jurkat using 83543-7-RR

    WB analysis of Jurkat using 83543-7-RR

    Jurkat cells were subjected to SDS PAGE followed by western blot with 83543-7-RR (CD5 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • IHC staining of human tonsillitis using 83543-7-RR

    IHC staining of human tonsillitis using 83543-7-RR

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 83543-7-RR (CD5 antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human tonsillitis using 83543-7-RR

    IHC staining of human tonsillitis using 83543-7-RR

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 83543-7-RR (CD5 antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Affinity and Kinetic Characterization of 83543-7-RR

    Affinity and Kinetic Characterization of 83543-7-RR

    Biolayer interferometry (BLl) kinetic assays of 83543-7-RR against Human CD5 were performed. The affinity constant is 2.09 nM.

经过测试的应用

Positive WB detected inJurkat cells
Positive IHC detected inhuman tonsillitis tissue, human ovary cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

The immunogen of 83543-7-RR is CD5 Fusion Protein expressed in E. coli.

Tested Applications WB, IHC, ELISA Application Description
Tested Reactivity human
Immunogen Fusion Protein 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name CD5 molecule
Synonyms T1, Lymphocyte antigen T1/Leu-1, Lymphocyte antigen T1/Leu 1, LEU1, CD5 molecule
Calculated Molecular Weight55 kDa
Observed Molecular Weight60 kDa
GenBank Accession NumberNM_014207.4
Gene Symbol CD5
Gene ID (NCBI) 921
ENSEMBL Gene IDENSG00000110448
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDP06127
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CD5 is a type I transmembrane glycoprotein of the scavenger receptor cysteine-rich family (PMID: 12403363). CD5 is expressed on a majority of thymocytes, mature T cells, B cell subsets, and peripheral blood dendritic cells (PMID: 9858516; 6156984; 10379049; 1384337). CD5 may act as a receptor in regulating T-cell proliferation. It functions as a negative regulator of TCR signaling during thymocyte development (PMID: 7542801).

实验方案

Product Specific Protocols
WB protocol for CD5 antibody 83543-7-RRDownload protocol
IHC protocol for CD5 antibody 83543-7-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols