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TIGIT Recombinant antibody

TIGIT Uni-rAbTM Recombinant Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

240423D3

Cat no : 83545-1-RR

Print datasheet

Synonyms

VSTM3, VSIG9, V-set and transmembrane domain-containing protein 3, V-set and immunoglobulin domain-containing protein 9, T-cell immunoreceptor with Ig and ITIM domains

验证数据展示

  • WB analysis using 83545-1-RR

    WB analysis using 83545-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 83545-1-RR (TIGIT antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • IHC staining of human tonsillitis using 83545-1-RR

    IHC staining of human tonsillitis using 83545-1-RR

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 83545-1-RR (TIGIT antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of Jurkat using 83545-1-RR

    IF Staining of Jurkat using 83545-1-RR

    Immunofluorescent analysis of (4% PFA) fixed Jurkat cells using TIGIT antibody (83545-1-RR, Clone: 240423D3 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • Affinity and Kinetic Characterization of 83545-1-RR

    Affinity and Kinetic Characterization of 83545-1-RR

    Biolayer interferometry (BLl) kinetic assays of 83545-1-RR against Human TIGIT were performed. The affinity constant is below 1 pM.

经过测试的应用

Positive WB detected inJurkat cells, NK-92 cells, NIH/3t3 cells, RAW 264.7 cells, EL-4 cells, rat spleen tissue
Positive IHC detected inhuman tonsillitis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inJurkat cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)/ICCIF/ICC : 1:125-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

The immunogen of 83545-1-RR is TIGIT Fusion Protein expressed in E. coli.

Tested Applications WB, IHC, IF/ICC, ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen Fusion Protein 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name T cell immunoreceptor with Ig and ITIM domains
Synonyms VSTM3, VSIG9, V-set and transmembrane domain-containing protein 3, V-set and immunoglobulin domain-containing protein 9, T-cell immunoreceptor with Ig and ITIM domains
Calculated Molecular Weight26 kDa
Observed Molecular Weight44 kDa
GenBank Accession NumberNM_173799.4
Gene Symbol TIGIT
Gene ID (NCBI) 201633
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ495A1-1
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

TIGIT (T-cell immunoreceptor with Ig and ITIM domains), also known as VSIG9 or VSTM3, is an immune receptor expressed on T cells, including Treg and memory subsets, as well as on NK cells (PMID: 19011627). It contains an immunoglobulin variable domain, a transmembrane domain and an immunoreceptor tyrosine-based inhibitory motif (ITIM). TIGIT binds to poliovirus receptor (PVR, also called CD155) with high affinity, and also to PVRL2 (CD112) with lower affinity. The interaction of TIGIT with PVR on dendritic cells increases the secretion of IL-10 and decreases the secretion of proinflammatory cytokine and suppresses T-cell activation by promoting the generation of mature immunoregulatory dendritic cells. TIGIT can inhibit NK cytotoxicity directly through its ITIM (PMID: 19815499).

实验方案

Product Specific Protocols
WB protocol for TIGIT antibody 83545-1-RRDownload protocol
IHC protocol for TIGIT antibody 83545-1-RRDownload protocol
IF protocol for TIGIT antibody 83545-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols