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MAGI2 Recombinant antibody

MAGI2 Uni-rAbTM Recombinant Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, rat

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

240512C10

Cat no : 83561-6-RR

Print datasheet

Synonyms

AIP-1, AIP1, AIP 1, ACVRIP1, ACVRINP1

验证数据展示

  • WB analysis of rat brain using 83561-6-RR

    WB analysis of rat brain using 83561-6-RR

    rat brain tissue were subjected to SDS PAGE followed by western blot with 83561-6-RR (MAGI2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Affinity and Kinetic Characterization of 83561-6-RR

    Affinity and Kinetic Characterization of 83561-6-RR

    Biolayer interferometry (BLl) kinetic assays of 83561-6-RR against Human MAGI2 were performed. The affinity constant is 1.90 nM.

经过测试的应用

Positive WB detected inrat brain tissue

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

The immunogen of 83561-6-RR is MAGI2 Fusion Protein expressed in E. coli.

Tested Applications WB, ELISA Application Description
Tested Reactivity human, rat
Immunogen MAGI2 fusion protein Ag18279 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name membrane associated guanylate kinase, WW and PDZ domain containing 2
Synonyms AIP-1, AIP1, AIP 1, ACVRIP1, ACVRINP1
Calculated Molecular Weight 1455 aa, 159 kDa
Observed Molecular Weight 140-150 kDa
GenBank Accession NumberBC150277
Gene Symbol MAGI2
Gene ID (NCBI) 9863
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDQ86UL8
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

MAGI proteins are scaffolding proteins, belonging to the Membrane-Associated Guanylate Kinase Inverted proteins of the MAGUK family. There are three members of the MAGI subfamily, MAGI-1, MAGI-2, and MAGI-3. They are comprised of 6 PDZ domains, 2 WW domains, and 1 GUK domain. They have been proven to mediate the transport and signal transduction of various G protein-coupled receptors (GPCRs) (PMID: 29625175). The antibody is specific to MAGI2.

实验方案

Product Specific Protocols
WB protocol for MAGI2 antibody 83561-6-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols