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验证数据展示

  • WB analysis of NIH/3T3 using 83562-3-RR

    WB analysis of NIH/3T3 using 83562-3-RR

    NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 83562-3-RR (Gsdmd antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • IF Staining of NIH/3T3 using 83562-3-RR

    IF Staining of NIH/3T3 using 83562-3-RR

    Immunofluorescent analysis of (-20°C Ethanol) fixed NIH/3T3 cells using Gsdmd antibody (83562-3-RR, Clone: 240548B10 ) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002).

  • FC experiment of RAW 264.7 using 83562-3-RR

    FC experiment of RAW 264.7 using 83562-3-RR

    1x10^6 RAW 264.7 cells were intracellularly stained with 0.25 ug Gsdmd Recombinant antibody (83562-3-RR, Clone:240548B10) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer.

  • Affinity and Kinetic Characterization of 83562-3-RR

    Affinity and Kinetic Characterization of 83562-3-RR

    Biolayer interferometry (BLl) kinetic assays of 83562-3-RR against Mouse Gsdmd were performed. The affinity constant is 1.19 nM.

GSDMD Recombinant monoclonal antibody

GSDMD Uni-rAb® Recombinant Antibody for WB, IF/ICC, FC (Intra), ELISA
Cat No. 83562-3-RR

产品说明书

CloneNo. 240548B10

宿主/亚型

Rabbit / IgG

种属反应性

mouse

应用

WB, IF/ICC, FC (Intra), ELISA

别名

240548B10, AW558049, DF5L, Dfna5l, gasdermin D

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inNIH/3T3 cells, EL-4 cells, RAW 264.7 cells, J774A.1 cells
Positive IF/ICC detected inNIH/3T3 cells
Positive FC (Intra) detected inRAW 264.7 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:6000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

83562-3-RR targets GSDMD in WB, IF/ICC, FC (Intra), ELISA applications and shows reactivity with mouse samples.

经测试应用 WB, IF/ICC, FC (Intra), ELISA Application Description
经测试反应性 mouse
免疫原

CatNo: Ag34905

Product name: Recombinant mouse Gsdmd protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 199-487 aa of BC029813

Sequence: GHQSRKKMVTIPAGSILAFRVAQLLIGSKWDILLVSDEKQRTFEPSSGDRKAVGQRHHGLNVLAALCSIGKQLSLLSDGIDEEELIEAADFQGLYAEVKACSSELESLEMELRQQILVNIGKILQDQPSMEALEASLGQGLCSGGQVEPLDGPAGCILECLVLDSGELVPELAAPIFYLLGALAVLSETQQQLLAKALETTVLSKQLELVKHVLEQSTPWQEQSSVSLPTVLLGDCWDEKNPTWVLLEECGLRLQVESPQVHWEPTSLIPTSALYASLFLLSSLGQKPC*

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 gasdermin D
别名 240548B10, AW558049, DF5L, Dfna5l, gasdermin D
计算分子量53 kDa
观测分子量53 kDa
GenBank蛋白编号BC029813
基因名称 Gsdmd
Gene ID (NCBI) 69146
RRIDAB_3671177
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDQ9D8T2
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

GSDMD is a 53 kDa cytosolic protein and a member of the gasdermin protein family, which features 6 members in humans (GSDMA, -B, -C, -D, -E and -F. GSDMD was identified as the executioner of inflammasome-associated pyroptosis. Caspase-1, -11, or -4 cleave GSDMD after an aspartate residue within a central linker domain (D275 in humans and D276 in mice) to generate a 31-kDa N-terminal GSDMD fragment (GSDMD-NT) and a 22-kDa C-terminal fragment (GSDMD-CT). In addition to the canonical 31/22 kDa cleavage products, a 43 kDa fragment has been observed under specific experimental or pathological conditions. The generation of the 43 kDa fragment was observed upon caspase-3 and -7 activation during apoptosis. This antibody can only detect full-length GSDMD.

实验方案

Product Specific Protocols
FC protocol for GSDMD antibody 83562-3-RRDownload protocol
IF protocol for GSDMD antibody 83562-3-RRDownload protocol
WB protocol for GSDMD antibody 83562-3-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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