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验证数据展示

  • WB analysis using 83565-1-RR

    WB analysis using 83565-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 83565-1-RR (MRPL51 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • WB analysis of HEK-293 using 83565-1-RR

    WB analysis of HEK-293 using 83565-1-RR

    WB result of MRPL51 antibody (83565-1-RR; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-MRPL51 transfected HEK-293 cells.

  • IHC staining of human lung cancer using 83565-1-RR

    IHC staining of human lung cancer using 83565-1-RR

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 83565-1-RR (MRPL51 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human ovarian cancer using 83565-1-RR

    IHC staining of human ovarian cancer using 83565-1-RR

    Immunohistochemical analysis of paraffin-embedded human ovarian cancer slide using 83565-1-RR (MRPL51 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of HEK-293 using 83565-1-RR

    IF Staining of HEK-293 using 83565-1-RR

    Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 cells using MRPL51 antibody (83565-1-RR, Clone: 240381C3 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

  • Affinity and Kinetic Characterization of 83565-1-RR

    Affinity and Kinetic Characterization of 83565-1-RR

    Biolayer interferometry (BLl) kinetic assays of 83565-1-RR against Human MRPL51 were performed. The affinity constant is 0.57 nM.

  • Featured Product
  • KD/KO验证

MRPL51 Recombinant antibody

MRPL51 Uni-rAbTM Recombinant Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 83565-1-RR

产品说明书

CloneNo. 240381C3

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, IF/ICC, ELISA

别名

HSPC241, CDA09, bMRP-64, bMRP64, bMRP 64

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHUVEC cells, HEK-293 cells, SKOV-3 cells
Positive IHC detected inhuman lung cancer tissue, human ovarian cancer
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHEK-293 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:200-1:800
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

83565-1-RR targets MRPL51 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
经测试反应性 human
免疫原 MRPL51 fusion protein Ag7193 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 mitochondrial ribosomal protein L51
别名 HSPC241, CDA09, bMRP-64, bMRP64, bMRP 64
计算分子量 15 kDa
观测分子量12 kDa
GenBank蛋白编号BC000191
基因名称 MRPL51
Gene ID (NCBI) 51258
RRIDAB_3671180
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purfication
UNIPROT IDQ4U2R6
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Mitochondrial ribosome protein L51 (MRPL51) is a 39S subunit protein of the mitochondrial ribosome. MRPL51 functions in the translation of genetic messages (PMID: 21730110). MRPL51 expression was positively correlated with cell cycle, DNA damage, DNA repair, epithelial-mesenchymal transition, invasion, and proliferation of LUAD cells at the singlecell level (PMID: 37323822). The calculated molecular weight of MRPL51 is 15 kDa.

实验方案

Product Specific Protocols
WB protocol for MRPL51 antibody 83565-1-RRDownload protocol
IHC protocol for MRPL51 antibody 83565-1-RRDownload protocol
IF protocol for MRPL51 antibody 83565-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols