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验证数据展示

  • IHC staining of human skin cancer using 83577-1-RR

    IHC staining of human skin cancer using 83577-1-RR

    Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 83577-1-RR (GAR1 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of HepG2 using 83577-1-RR

    IF Staining of HepG2 using 83577-1-RR

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using GAR1 antibody (83577-1-RR, Clone: 240537C11 ) at dilution of 1:300 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

  • FC experiment of K562 using 83577-1-RR

    FC experiment of K562 using 83577-1-RR

    1x10^6 K562 were intracellularly stained with 0.25 ug GAR1 Recombinant antibody (83577-1-RR, Clone:240537C11) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

  • Affinity and Kinetic Characterization of 83577-1-RR

    Affinity and Kinetic Characterization of 83577-1-RR

    Biolayer interferometry (BLl) kinetic assays of 83577-1-RR against Human GAR1 were performed. The affinity constant is below 1 pM.

GAR1 Recombinant antibody

GAR1 Uni-rAbTM Recombinant Antibody for IHC, IF/ICC, ELISA
Cat No. 83577-1-RR

产品说明书

CloneNo. 240537C11

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

IHC, IF/ICC, ELISA

别名

snoRNP protein GAR1, NOLA1, 240537C11

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
CoraLite® Plus 488
规格: 

-/ -

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经过测试的应用

Positive IHC detected inhuman skin cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells
Positive FC (Intra) detected inK562

推荐稀释比

应用推荐稀释比
Immunohistochemistry (IHC)IHC : 1:250-1:1000
Immunofluorescence (IF)/ICCIF/ICC : 1:150-1:600
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

83577-1-RR targets GAR1 in IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

经测试应用 IHC, IF/ICC, ELISA Application Description
经测试反应性 human
免疫原 GAR1 fusion protein Ag2282 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 GAR1 ribonucleoprotein homolog (yeast)
别名 snoRNP protein GAR1, NOLA1, 240537C11
计算分子量 217 aa, 22 kDa
GenBank蛋白编号BC003413
基因名称 GAR1
Gene ID (NCBI) 54433
RRIDAB_3671188
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ9NY12
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

GAR1, other named NOLA1, is a subunit of H/ACA and telomerase. Thought is not required for H/ACA protein assembly, GAR1 is necessary for ribosome biogenesis and telomere. H/ACA involves in class specify the sites of uridine-to-pseudouridine. It contains a core domain that flanked by glycine- and arginine-rich(GAR) domains. GAR1 also required for correct processing or intranuclear trafficking of TERC, the RNA component of the telomerase reverse transciptase(TERT) holoenzyme.

实验方案

Product Specific Protocols
IHC protocol for GAR1 antibody 83577-1-RRDownload protocol
IF protocol for GAR1 antibody 83577-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols