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PD-L1/CD274 Recombinant antibody, PBS Only (Capture)

PD-L1/CD274 Recombinant Antibody for Sandwich ELISA, Indirect ELISA, Sample test

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

Sandwich ELISA, Indirect ELISA, Sample test

Conjugate

Unconjugated

CloneNo.

240721D4

Cat No : 83600-7-PBS

Print datasheet

Synonyms

CD274, PD-L1, PD L1, hPD-L1, B7-H1

验证数据展示

  • Sandwich ELISA standard curve of MP00601-4

    Sandwich ELISA standard curve of MP00601-4

    Sandwich ELISA standard curve of MP00601-4, Human PD-L1/CD274 Recombinant Matched Antibody Pair - PBS only. 83600-7-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Eg1114. 83600-6-PBS was HRP conjugated as the detection antibody. Range: 3.9-250 pg/mL.

  • Sample test of MP00601-4

    Sample test of MP00601-4

    Human peripheral blood mononuclear cells (PBMC) were cultured unstimulated or stimulated with 10 μg/mL PHA for 3 days or 5 days. For the PBMC supernatant cultured for 3 days, the mean PD-L1/CD274 concentration was determined to be 7.7 pg/mL in unstimulated PBMC supernatant, 108.4 pg/mL in PHA stimulated PBMC supernatant. For the PBMC supernatant cultured for 5 days, the mean PD-L1/CD274 concentration was determined to be 11.0 pg/mL in unstimulated PBMC supernatant, 160.7 pg/mL in PHA stimulated PBMC supernatant.

  • Sample test of MP00601-4

    Sample test of MP00601-4

    Urine of seven individual healthy human donors was measured. The PD-L1/CD274 concentration of detected samples was determined to be 10.8 pg/mL with a range of 0.7 - 33.1 pg/mL.

  • Sample test of MP00601-4

    Sample test of MP00601-4

    The mean PD-L1/CD274 concentration was determined to be 36,368.3 pg/mL in MDA-MB-231 cell extract based on a 2.3 mg/mL extract load.

  • Sample test of MP00601-4

    Sample test of MP00601-4

    Serum of sixteen individual healthy human donors was measured. The human PD-L1/CD274 concentration of detected samples was determined to be 75.1 pg/mL with a range of 34.4-168.1 pg/mL.

产品信息

83600-7-PBS targets PD-L1/CD274 as part of a matched antibody pair:

MP00601-4: 83600-7-PBS capture and 83600-6-PBS detection (validated in Sandwich ELISA)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Applications Sandwich ELISA, Indirect ELISA, Sample test Application Description
Tested Reactivity human
Immunogen Fusion Protein 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name CD274 molecule
Synonyms CD274, PD-L1, PD L1, hPD-L1, B7-H1
Calculated Molecular Weight 290 aa, 33 kDa
GenBank Accession NumberBC074984
Gene Symbol PD-L1
Gene ID (NCBI) 29126
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ9NZQ7
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C