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Conjugate
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CUL3 Recombinant antibody

CUL3 Recombinant Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

240534C7

Cat No : 83619-5-RR

Print datasheet

Synonyms

KIAA0617, Cullin-3, CUL-3, 240534C7

验证数据展示

  • WB analysis using 83619-5-RR

    WB analysis using 83619-5-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 83619-5-RR (CUL3 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • Affinity and Kinetic Characterization of 83619-5-RR

    Affinity and Kinetic Characterization of 83619-5-RR

    Biolayer interferometry (BLl) kinetic assays of 83619-5-RR against Human CUL3 were performed. The affinity constant is below 1 pM.

经过测试的应用

Positive WB detected inLNCaP cells, SH-SY5Y cells, PC-12 cells
Planning an IHC experiment? We recommend our IHCeasy CUL3 Ready-To-Use IHC Kit. CUL3 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

The immunogen of 83619-5-RR is CUL3 Fusion Protein expressed in E. coli.

Tested Applications WB, ELISA Application Description
Tested Reactivity human
Immunogen CUL3 fusion protein Ag1555 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name cullin 3
Synonyms KIAA0617, Cullin-3, CUL-3, 240534C7
Calculated Molecular Weight 89 kDa
Observed Molecular Weight 80-89 kDa
GenBank Accession NumberBC039598
Gene Symbol CUL3
Gene ID (NCBI) 8452
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDQ13618
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Cullin-RING-based BCR (BTB-CUL3-RBX1) E3 ligase are the largest family of ubiquitin ligases which mediate the ubiquitination and subsequent proteasomal degradation of target proteins. One of seven cullin proteins serves as a scaffold protein for the assembly of the multisubunit ubiquitin ligase complex. CUL3 (Cullin-3) ubiquitin ligase complex targets multiple substrate for ubiquitination including cyclin E and of cyclin D1. Defects in CUL3 are the cause of Pseudohypoaldosteronism type 2E (PHA2E) characterized by severe hypertension, hyperkalemia, hyperchloremia, hyperchloremic metabolic acidosis, and correction of physiologic abnormalities by thiazide diuretics.

实验方案

Product Specific Protocols
WB protocol for CUL3 antibody 83619-5-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols