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验证数据展示

  • WB analysis using 83637-4-RR

    WB analysis using 83637-4-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 83637-4-RR (Synaptotagmin-1 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse brain using 83637-4-RR

    IHC staining of mouse brain using 83637-4-RR

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 83637-4-RR (Synaptotagmin-1 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with SYT1 antibody included
  • IF Staining of SH-SY5Y using 83637-4-RR

    IF Staining of SH-SY5Y using 83637-4-RR

    Immunofluorescent analysis of (4% PFA) fixed SH-SY5Y cells using Synaptotagmin-1 antibody (83637-4-RR, Clone: 240589F6 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • IF Staining of SH-SY5Y using 83637-4-RR

    IF Staining of SH-SY5Y using 83637-4-RR

    Immunofluorescent analysis of (4% PFA) fixed SH-SY5Y cells using Synaptotagmin-1 antibody (83637-4-RR, Clone: 240589F6 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • Affinity and Kinetic Characterization of 83637-4-RR

    Affinity and Kinetic Characterization of 83637-4-RR

    Biolayer interferometry (BLl) kinetic assays of 83637-4-RR against Human Synaptotagmin-1 were performed. The affinity constant is 20.5 nM.

Synaptotagmin-1 Recombinant antibody

Synaptotagmin-1 Uni-rAbTM Recombinant Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 83637-4-RR

产品说明书

CloneNo. 240589F6

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, ELISA

别名

Synaptotagmin 1, SYT1, SYT, synaptotagmin I, SVP65

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
CoraLite® Plus 488
规格: 

-/ -

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经过测试的应用

Positive WB detected inmouse brain tissue, mouse cerebellum tissue, rat brain tissue
Positive IHC detected inmouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inSH-SY5Y cells
Planning an IHC experiment? We recommend our IHCeasy SYT1 Ready-To-Use IHC Kit. SYT1 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:2000-1:16000
Immunohistochemistry (IHC)IHC : 1:1000-1:4000
Immunofluorescence (IF)/ICCIF/ICC : 1:125-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

83637-4-RR targets Synaptotagmin-1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 Synaptotagmin-1 fusion protein Ag5970 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 synaptotagmin I
别名 Synaptotagmin 1, SYT1, SYT, synaptotagmin I, SVP65
计算分子量 48 kDa
观测分子量 60 kDa
GenBank蛋白编号BC058917
基因名称 Synaptotagmin-1
Gene ID (NCBI) 6857
RRIDAB_3671249
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purfication
UNIPROT IDP21579
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

The synaptotagmins are integral membrane proteins of synaptic vesicles thought to serve as Ca(2+) sensors in the process of vesicular trafficking and exocytosis (PMID: 8058779). SYT1 (synaptotagmin I) was firstly identified as a 65-kDa protein with a wide distribution in neuronal and neurosecretory tissue (PMID: 7298720). Calcium binding to SYT1 participates in triggering neurotransmitter release at the synapse (PMID: 11242035). In addition to regulating exocytosis, SYT1 has also been implicated in endocytosis and neurite outgrowth (PMID: 15492212; 11078930; 10336114). This antibody recognizes endogenous SYT1, which has an experimentally determined molecular mass of 65-68 kDa. An additional band of 47 kDa could also be detected, which is corresponding to the calculated molecular weight of SYT1.

实验方案

Product Specific Protocols
WB protocol for Synaptotagmin-1 antibody 83637-4-RRDownload protocol
IHC protocol for Synaptotagmin-1 antibody 83637-4-RRDownload protocol
IF protocol for Synaptotagmin-1 antibody 83637-4-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols