• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

验证数据展示

  • WB analysis using 83649-5-RR

    WB analysis using 83649-5-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 83649-5-RR (Involucrin antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IHC staining of human skin using 83649-5-RR

    IHC staining of human skin using 83649-5-RR

    Immunohistochemical analysis of paraffin-embedded human skin tissue slide using 83649-5-RR (Involucrin antibody) at dilution of 1:800 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human skin cancer using 83649-5-RR

    IHC staining of human skin cancer using 83649-5-RR

    Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 83649-5-RR (Involucrin antibody) at dilution of 1:800 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Affinity and Kinetic Characterization of 83649-5-RR

    Affinity and Kinetic Characterization of 83649-5-RR

    Biolayer interferometry (BLl) kinetic assays of 83649-5-RR against Human Involucrin were performed. The affinity constant is below 1 pM.

Involucrin Recombinant antibody

Involucrin Recombinant Antibody for WB, IHC, ELISA
Cat No. 83649-5-RR

产品说明书

CloneNo. 240566D8

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, ELISA

别名

IVL, 240566D8

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

点击咨询售前客服

点击咨询售后客服

经过测试的应用

Positive WB detected inA431 cells, HaCaT cells, HT-29 cells, HT-1080 cells
Positive IHC detected inhuman skin tissue, human skin cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

83649-5-RR targets Involucrin in WB, IHC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human
免疫原 Involucrin fusion protein Ag29585 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 involucrin
别名 IVL, 240566D8
计算分子量 68 kDa
观测分子量140 kDa
GenBank蛋白编号NM_005547
基因名称 Involucrin
Gene ID (NCBI) 3713
RRIDAB_3671258
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purfication
UNIPROT IDP07476
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Involucrin is a protein precursor of the epidermal cornified envelope that is assembled in the outermost layers of the epidermis. Involucrin expression is restricted to the suprabasal epidermal layers (spinous and granular layers) during normal keratinocyte differentiation and is a useful marker of terminal differentiation. The predicted MW of involucrin is 68 kDa, but it also forms 120 kDa dimers, while different reports provided variable results ranging from 90-140 kDa. (PMID: 11099111, 1503502, 12150517)

实验方案

Product Specific Protocols
WB protocol for Involucrin antibody 83649-5-RRDownload protocol
IHC protocol for Involucrin antibody 83649-5-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols