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RNASET2 Recombinant antibody

RNASET2 Recombinant Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

240581C4

Cat No : 83650-1-RR

Print datasheet

Synonyms

RNase T2, Ribonuclease T2, Ribonuclease 6, EC:4.6.1.19, bA514O12.3

验证数据展示

  • WB analysis using 83650-1-RR

    WB analysis using 83650-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 83650-1-RR (RNASET2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Affinity and Kinetic Characterization of 83650-1-RR

    Affinity and Kinetic Characterization of 83650-1-RR

    Biolayer interferometry (BLl) kinetic assays of 83650-1-RR against Human RNASET2 were performed. The affinity constant is 2.09 nM.

经过测试的应用

Positive WB detected inK-562 cells, HL-60 cells, THP-1 cells
Planning an IHC experiment? We recommend our IHCeasy RNASET2 Ready-To-Use IHC Kit. RNASET2 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

The immunogen of 83650-1-RR is RNASET2 Fusion Protein expressed in E. coli.

Tested Applications WB, ELISA Application Description
Tested Reactivity human
Immunogen RNASET2 fusion protein Ag28353 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name ribonuclease T2
Synonyms RNase T2, Ribonuclease T2, Ribonuclease 6, EC:4.6.1.19, bA514O12.3
Calculated Molecular Weight 256 aa, 29 kDa
Observed Molecular Weight25-45 kDa
GenBank Accession NumberBC039713
Gene Symbol RNase T2
Gene ID (NCBI) 8635
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDO00584
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

RNASET2(Ribonuclease T2) is also named as RNASE6PL(Ribonuclease 6) and belongs to the RNase T2 family. It is a unique member of the growing family of tumor-antagonizing genes and behaves as a class II tumor suppressor and abolish the tumorigenic potential of an ovarian cancer-derived cell line. This protein has 2 isoforms produced by alternative splicing. Both mildly glycosylated (ranging from38 to 45 kDa) and highly glycosylated forms (ranging from 50 to 80 kDa) of human tumor suppressor protein RNASET2 can be detected in immunoblotting in P. Pastoris(PMID: 21446958).

实验方案

Product Specific Protocols
WB protocol for RNASET2 antibody 83650-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols