• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

验证数据展示

  • WB analysis using 83664-2-RR

    WB analysis using 83664-2-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 83664-2-RR (FTCD antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

  • FC experiment of HepG2 using 83664-2-RR

    FC experiment of HepG2 using 83664-2-RR

    1x10^6 HepG2 cells were intracellularly stained with 0.25 ug FTCD Recombinant antibody (83664-2-RR, Clone:240654G9) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • Affinity and Kinetic Characterization of 83664-2-RR

    Affinity and Kinetic Characterization of 83664-2-RR

    Biolayer interferometry (BLl) kinetic assays of 83664-2-RR against Human FTCD were performed. The affinity constant is 6.37 nM.

FTCD Recombinant antibody

FTCD Recombinant Antibody for WB, ELISA
Cat No. 83664-2-RR

产品说明书

CloneNo. 240654G9

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, ELISA

别名

Glutamate formiminotransferase, Glutamate formimidoyltransferase, Formiminotransferase-cyclodeaminase, Formimidoyltransferase-cyclodeaminase, Formimidoyltetrahydrofolate cyclodeaminase

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

点击咨询售前客服

点击咨询售后客服

经过测试的应用

Positive WB detected inmouse liver tissue, rat liver tissue, human kidney tissue
Positive FC (Intra) detected inHepG2 cells
Planning an IHC experiment? We recommend our IHCeasy FTCD Ready-To-Use IHC Kit. FTCD primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:2000-1:12000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

83664-2-RR targets FTCD in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 FTCD fusion protein Ag16646 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 formiminotransferase cyclodeaminase
别名 Glutamate formiminotransferase, Glutamate formimidoyltransferase, Formiminotransferase-cyclodeaminase, Formimidoyltransferase-cyclodeaminase, Formimidoyltetrahydrofolate cyclodeaminase
计算分子量 541 aa, 59 kDa
观测分子量58 kDa
GenBank蛋白编号BC136395
基因名称 FTCD
Gene ID (NCBI) 10841
RRIDAB_3671271
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDO95954
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

FTCD (formimidoyltransferase cyclodeaminase) is critical for the catabolism of histidine, a . This process is one-carbon units that can enter the one-carbon/folate cycle, which provides methyl groups for arsenic metabolism (PMID: 30893314). In addition, FTCD could serve as a predictive and prognostic marker for patients with HCC (PMID: 37675273).

实验方案

Product Specific Protocols
WB protocol for FTCD antibody 83664-2-RRDownload protocol
FC protocol for FTCD antibody 83664-2-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols