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UQCRC1 Recombinant antibody

UQCRC1 Uni-rAbTM Recombinant Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

240507A6

Cat no : 83671-2-RR

Print datasheet

Synonyms

QCR1, D3S3191, Cytochrome b-c1 complex subunit 1, mitochondrial, Core protein I, Complex III subunit 1

验证数据展示

  • WB analysis using 83671-2-RR

    WB analysis using 83671-2-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 83671-2-RR (UQCRC1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IHC staining of human ovary cancer using 83671-2-RR

    IHC staining of human ovary cancer using 83671-2-RR

    Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 83671-2-RR (UQCRC1 antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with UQCRC1 antibody included
  • Affinity and Kinetic Characterization of 83671-2-RR

    Affinity and Kinetic Characterization of 83671-2-RR

    Biolayer interferometry (BLl) kinetic assays of 83671-2-RR against Human UQCRC1 were performed. The affinity constant is 0.294 nM.

经过测试的应用

Positive WB detected inHeLa cells, L02 cells, HepG2 cells, mouse brain tissue, mouse liver tissue, rat brain tissue, rat liver tissue
Positive IHC detected inhuman ovary cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy UQCRC1 Ready-To-Use IHC Kit. UQCRC1 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

The immunogen of 83671-2-RR is UQCRC1 Fusion Protein expressed in E. coli.

Tested Applications WB, IHC, ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen UQCRC1 fusion protein Ag16322 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name ubiquinol-cytochrome c reductase core protein I
Synonyms QCR1, D3S3191, Cytochrome b-c1 complex subunit 1, mitochondrial, Core protein I, Complex III subunit 1
Calculated Molecular Weight 480 aa, 53 kDa
Observed Molecular Weight 45 kDa
GenBank Accession NumberBC009586
Gene Symbol UQCRC1
Gene ID (NCBI) 7384
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDP31930
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

UQCRC1(Cytochrome b-c1 complex subunit 1, mitochondrial), also named as QCR1 and UQCR1, is a ubunit of the cytochrome bc1 complex (complex III) of the mitochondrial respiratory chain, may mediate the formation of the complex between cytochromes c and c1. The gene encodes a 53 kDa protein with a 34 amino acids transit peptide. It is associated with apoptosis or inhibition of cancer cell growth.

实验方案

Product Specific Protocols
WB protocol for UQCRC1 antibody 83671-2-RRDownload protocol
IHC protocol for UQCRC1 antibody 83671-2-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols