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验证数据展示

  • WB analysis using 83671-2-RR

    WB analysis using 83671-2-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 83671-2-RR (UQCRC1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IHC staining of human stomach cancer using 83671-2-RR

    IHC staining of human stomach cancer using 83671-2-RR

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 83671-2-RR (UQCRC1 antibody) at dilution of 1:2000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer(pH9).

    Ready-to-use IHC kits available with UQCRC1 antibody included
  • Affinity and Kinetic Characterization of 83671-2-RR

    Affinity and Kinetic Characterization of 83671-2-RR

    Biolayer interferometry (BLl) kinetic assays of 83671-2-RR against Human UQCRC1 were performed. The affinity constant is 0.294 nM.

UQCRC1 Recombinant antibody

UQCRC1 Recombinant Antibody for WB, IHC, ELISA
Cat No. 83671-2-RR

产品说明书

CloneNo. 240507A6

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, ELISA

别名

QCR1, D3S3191, Cytochrome b-c1 complex subunit 1, mitochondrial, Core protein I, Complex III subunit 1

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHeLa cells, L02 cells, HepG2 cells, mouse brain tissue, mouse liver tissue, rat brain tissue, rat liver tissue
Positive IHC detected inhuman stomach cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy UQCRC1 Ready-To-Use IHC Kit. UQCRC1 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:1000-1:4000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

83671-2-RR targets UQCRC1 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 UQCRC1 fusion protein Ag16322 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 ubiquinol-cytochrome c reductase core protein I
别名 QCR1, D3S3191, Cytochrome b-c1 complex subunit 1, mitochondrial, Core protein I, Complex III subunit 1
计算分子量 480 aa, 53 kDa
观测分子量 45 kDa
GenBank蛋白编号BC009586
基因名称 UQCRC1
Gene ID (NCBI) 7384
RRIDAB_3671276
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purfication
UNIPROT IDP31930
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

UQCRC1(Cytochrome b-c1 complex subunit 1, mitochondrial), also named as QCR1 and UQCR1, is a ubunit of the cytochrome bc1 complex (complex III) of the mitochondrial respiratory chain, may mediate the formation of the complex between cytochromes c and c1. The gene encodes a 53 kDa protein with a 34 amino acids transit peptide. It is associated with apoptosis or inhibition of cancer cell growth.

实验方案

Product Specific Protocols
WB protocol for UQCRC1 antibody 83671-2-RRDownload protocol
IHC protocol for UQCRC1 antibody 83671-2-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols