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验证数据展示

  • WB analysis using 83675-6-RR

    WB analysis using 83675-6-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 83675-6-RR (SCTR antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IF Staining of THP-1 using 83675-6-RR

    IF Staining of THP-1 using 83675-6-RR

    Immunofluorescent analysis of (4% PFA) fixed THP-1 cells using SCTR antibody (83675-6-RR, Clone: 240675E6 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • Affinity and Kinetic Characterization of 83675-6-RR

    Affinity and Kinetic Characterization of 83675-6-RR

    Biolayer interferometry (BLl) kinetic assays of 83675-6-RR against Human SCTR were performed. The affinity constant is 6.05 nM.

SCTR Recombinant antibody

SCTR Recombinant Antibody for WB, IF/ICC, ELISA
Cat No. 83675-6-RR

产品说明书

CloneNo. 240675E6

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IF/ICC, ELISA

别名

SR, secretin receptor, SCT-R, SCT R, 240675E6

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inTHP-1 cells, mouse pancreas tissue
Positive IF/ICC detected inTHP-1 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

83675-6-RR targets SCTR in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IF/ICC, ELISA Application Description
经测试反应性 human
免疫原 SCTR fusion protein Ag5371 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 SCTR
别名 SR, secretin receptor, SCT-R, SCT R, 240675E6
计算分子量 440 aa, 50 kDa
观测分子量45-50 kDa
GenBank蛋白编号BC035757
基因名称 SCTR
Gene ID (NCBI) 6344
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purfication
UNIPROT IDP47872
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

SCTR is a member of the family B G protein-coupled receptors. SCTR is a receptor for SCT, a gastrointestinal peptide hormone secreted by the S cells of the duodenum. SCT regulates water homeostasis throughout the body and influences the environment of the duodenum by regulating SCT in the stomach and pancreas. Studies suggest that SCT can act as a neuropeptide within the central nervous system (CNS), thus SCTR may regulate the function of the CNS.

实验方案

Product Specific Protocols
WB protocol for SCTR antibody 83675-6-RRDownload protocol
IF protocol for SCTR antibody 83675-6-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols