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验证数据展示

  • WB analysis using 83677-2-RR

    WB analysis using 83677-2-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 83677-2-RR (PBRM1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • IF Staining of HeLa using 83677-2-RR

    IF Staining of HeLa using 83677-2-RR

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using PBRM1 antibody (83677-2-RR, Clone: 240655H5 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

  • Affinity and Kinetic Characterization of 83677-2-RR

    Affinity and Kinetic Characterization of 83677-2-RR

    Biolayer interferometry (BLl) kinetic assays of 83677-2-RR against Human PBRM1 were performed. The affinity constant is 2.34 nM.

PBRM1 Recombinant antibody

PBRM1 Uni-rAbTM Recombinant Antibody for WB, IF/ICC, ELISA
Cat No. 83677-2-RR

产品说明书

CloneNo. 240655H5

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IF/ICC, ELISA

别名

PBRM 1, PB1, hPB1, BRG1-associated factor 180, BAF180

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inJurkat cells, K-562 cells, HeLa cellls, U2OS cells, NIH/3T3 cells
Positive IF/ICC detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy PBRM1 Ready-To-Use IHC Kit. PBRM1 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:2000-1:10000
Immunofluorescence (IF)/ICCIF/ICC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

83677-2-RR targets PBRM1 in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IF/ICC, ELISA Application Description
经测试反应性 human, mouse
免疫原 PBRM1 fusion protein Ag33623 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 polybromo 1
别名 PBRM 1, PB1, hPB1, BRG1-associated factor 180, BAF180
计算分子量 180 kDa
观测分子量180-195 kDa
GenBank蛋白编号BC015323
基因名称 PBRM1
Gene ID (NCBI) 55193
RRIDAB_3671280
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ86U86
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

PBRM1, also named hPB1 BAF180 and Polybromo-1D, acts as a negative regulator of cell proliferation. PBRM1 is the second major mutated clear cell RCC cancer gene after VHL. PBRM1 loss is associated with low grade features.

实验方案

Product Specific Protocols
WB protocol for PBRM1 antibody 83677-2-RRDownload protocol
IF protocol for PBRM1 antibody 83677-2-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols