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验证数据展示

  • WB analysis using 83682-4-RR

    WB analysis using 83682-4-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 83682-4-RR (CDCA7L antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • FC experiment of A431 using 83682-4-RR

    FC experiment of A431 using 83682-4-RR

    1x10^6 A431 cells were intracellularly stained with 0.25 ug CDCA7L Recombinant antibody (83682-4-RR, Clone:240639A3) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

  • Affinity and Kinetic Characterization of 83682-4-RR

    Affinity and Kinetic Characterization of 83682-4-RR

    Biolayer interferometry (BLl) kinetic assays of 83682-4-RR against Human CDCA7L were performed. The affinity constant is 7.75 nM.

CDCA7L Recombinant antibody

CDCA7L Uni-rAbTM Recombinant Antibody for WB, FC (Intra), ELISA
Cat No. 83682-4-RR
CloneNo. 240639A3

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, FC (Intra), ELISA

Synonyms

R1, Protein JPO2, JPO2, HR1, Cell division cycle-associated 7-like protein

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size: 

-/ -

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经过测试的应用

Positive WB detected inA549 cells, HEK-293T cells, HeLa cells, K-562 cells, MCF-7 cells
Positive FC (Intra) detected inA431 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

83682-4-RR targets CDCA7L in WB, FC (Intra), ELISA applications and shows reactivity with human samples.

Tested Applications WB, FC (Intra), ELISA Application Description
Tested Reactivity human
Immunogen CDCA7L fusion protein Ag10944 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name cell division cycle associated 7-like
Synonyms R1, Protein JPO2, JPO2, HR1, Cell division cycle-associated 7-like protein
Calculated Molecular Weight 454 aa, 52 kDa
Observed Molecular Weight52 kDa
GenBank Accession NumberBC009352
Gene Symbol CDCA7L
Gene ID (NCBI) 55536
RRIDAB_3671283
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDQ96GN5
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Cell division cycle-associated 7-like protein (CDCA7L) is also known as HR1 and JPO2 and belongs to the JPO protein family. CDCA7L is identified as a target gene of c-Myc and is frequently dysregulated in multiple cancers. CDCA7L can complement the c-Myc transformation-defective mutant W135E and potentiate Myc-mediated transformation (PMID: 24141559, PMID: 37140023). CDCA7L translocates from cytoplasm to nucleus under dexamethasone induction. CDCA7L is widely expressed. CDCA7L has 4 isoforms produced by alternative splicing (refer to UniProt).

实验方案

Product Specific Protocols
WB protocol for CDCA7L antibody 83682-4-RRDownload protocol
FC protocol for CDCA7L antibody 83682-4-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols