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验证数据展示

  • WB analysis using 83713-2-RR

    WB analysis using 83713-2-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 83713-2-RR (CD34 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IF Staining of NIH/3T3 using 83713-2-RR

    IF Staining of NIH/3T3 using 83713-2-RR

    Immunofluorescent analysis of (4% PFA) fixed NIH/3T3 cells using CD34 antibody (83713-2-RR, Clone: 240659C7 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • IHC staining of mouse testis using 83713-2-RR

    IHC staining of mouse testis using 83713-2-RR

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 83713-2-RR (CD34 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Affinity and Kinetic Characterization of 83713-2-RR

    Affinity and Kinetic Characterization of 83713-2-RR

    Biolayer interferometry (BLl) kinetic assays of 83713-2-RR against Mouse CD34 were performed. The affinity constant is 1.09 nM.

CD34 Recombinant antibody

CD34 Recombinant Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 83713-2-RR

产品说明书

CloneNo. 240659C7

宿主/亚型

Rabbit / IgG

种属反应性

mouse

应用

WB, IHC, IF/ICC, ELISA

别名

CD34 antigen, 240659C7

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inC2C12 cells
Positive IHC detected inmouse testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inNIH/3T3 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)/ICCIF/ICC : 1:125-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

83713-2-RR targets CD34 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with mouse samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
经测试反应性 mouse
免疫原 Recombinant protein 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 CD34 antigen
别名 CD34 antigen, 240659C7
计算分子量41 kDa
观测分子量105 kDa
GenBank蛋白编号NM_133654
基因名称 CD34
Gene ID (NCBI) 12490
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ64314
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CD34 is a 105- to 120-kDa glycophosphoprotein expressed on the majority of hematopoietic stem/progenitor cells, bone marrow stromal cells, capillary endothelial cells, embryonic fibroblasts, and some nerve tissue. CD34 is a commonly used marker for identifying human hematopoietic stem/progenitor cells and mediates cell adhesion and lymphocyte homing by binding L-selectin and E-selectin ligands. CD34 is also one of the best negative selection markers for characterizing and/or isolating human MSCs from bone marrow and other sources. Along with other positive selection markers (such as CD29, CD44, CD90, CD105 and CD166), negative selection markers (such as CD34 and CD45) are used for MSC identification.

实验方案

Product Specific Protocols
WB protocol for CD34 antibody 83713-2-RRDownload protocol
IHC protocol for CD34 antibody 83713-2-RRDownload protocol
IF protocol for CD34 antibody 83713-2-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols