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MICA/MICB Recombinant antibody

MICA/MICB Recombinant Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

240735D9

Cat no : 83725-5-RR

Print datasheet

Synonyms

MICB, MICA, PERB11.1, MIC A, 240735D9

验证数据展示

  • WB analysis using 83725-5-RR

    WB analysis using 83725-5-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 83725-5-RR (MICA/MICB antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

  • Affinity and Kinetic Characterization of 83725-5-RR

    Affinity and Kinetic Characterization of 83725-5-RR

    Biolayer interferometry (BLl) kinetic assays of 83725-5-RR against Human MICA were performed. The affinity constant is 1.55 nM.

经过测试的应用

Positive WB detected inK-562 cells, HeLa cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:16000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

83725-5-RR targets MICA/MICB in WB, ELISA applications and shows reactivity with human samples.

Tested Applications WB, ELISA Application Description
Tested Reactivity human
Immunogen Recombinant protein 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name MHC class I polypeptide-related sequence A
Synonyms MICB, MICA, PERB11.1, MIC A, 240735D9
Calculated Molecular Weight43 kDa
Observed Molecular Weight50-60 kDa
GenBank Accession NumberAAH16929.1
Gene Symbol MICA
Gene ID (NCBI) 100507436
RRIDAB_3671325
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Human MHC class I chain-related genes located within the HLA class I region of chromosome 6 encode MHC class I chain-related A and B (MICA and MICB) (PMID: 11429322). MICA and MICB are stress-inducible surface molecules that are not associated with β2-microglobulin and do not present peptides (PMID: 9497295). They are expressed in intestinal epithelium and many epithelial tumors (PMID: 10359807). MICA and MICB are ligands for NKG2D which is an activating receptor that is expressed on most natural killer (NK) cells, CD8 αβ T cells, and γδ T cells (PMID: 10426993; 11491531). This antibody recognizes both MICA and MICB.

实验方案

Product Specific Protocols
WB protocol for MICA/MICB antibody 83725-5-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols