• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

验证数据展示

  • WB analysis using 83731-2-RR

    WB analysis using 83731-2-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 83731-2-RR (ACOX1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • FC experiment of A431 using 83731-2-RR

    FC experiment of A431 using 83731-2-RR

    1x10^6 A431 cells were intracellularly stained with 0.25 ug ACOX1 Recombinant antibody (83731-2-RR, Clone:240754E3) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • IHC staining of human ovarian cancer using 83731-2-RR

    IHC staining of human ovarian cancer using 83731-2-RR

    Immunohistochemical analysis of paraffin-embedded human ovarian cancer slide using 83731-2-RR (ACOX1 antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Affinity and Kinetic Characterization of 83731-2-RR

    Affinity and Kinetic Characterization of 83731-2-RR

    Biolayer interferometry (BLl) kinetic assays of 83731-2-RR against Human ACOX1 were performed. The affinity constant is below 1 pM.

ACOX1 Recombinant antibody

ACOX1 Recombinant Antibody for WB, IHC, FC (Intra), ELISA
Cat No. 83731-2-RR

产品说明书

CloneNo. 240754E3

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, FC (Intra), ELISA

别名

Palmitoyl-CoA oxidase, EC:1.3.3.6, AOX, ACOX 1, ACOX

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

点击咨询售前客服

点击咨询售后客服

经过测试的应用

Positive WB detected inmouse liver tissue, rat liver tissue
Positive IHC detected inhuman ovarian cancer
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive FC (Intra) detected inA431 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

83731-2-RR targets ACOX1 in WB, IHC, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, FC (Intra), ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 ACOX1 fusion protein Ag1405 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 acyl-Coenzyme A oxidase 1, palmitoyl
别名 Palmitoyl-CoA oxidase, EC:1.3.3.6, AOX, ACOX 1, ACOX
计算分子量 74 kDa
观测分子量70-74 kDa, 22 kDa
GenBank蛋白编号BC010425
基因名称 ACOX1
Gene ID (NCBI) 51
RRIDAB_3671331
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ15067
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

ACOX1(Peroxisomal acyl-coenzyme A oxidase 1) is the first and rate-limiting enzyme in the peroxisomal fatty acid beta-oxidation pathway and catalyzes the desaturation of acyl-CoAs to 2-trans-enoyl-CoAs. It belongs to the acyl-CoA oxidase family and also named as ACOX, AOX, SCOX. It has 3 isoforms produced by alternative splicing with the molecular mass of 70-74 kDa and can be cleaved post-translationally into a 50-kDa and a 22-kDa subunit between Val468 and Ala469 (PMID: 10672038). Defects in ACOX1 are the cause of adrenoleukodystrophy pseudoneonatal (Pseudo-NALD).

实验方案

Product Specific Protocols
WB protocol for ACOX1 antibody 83731-2-RRDownload protocol
IHC protocol for ACOX1 antibody 83731-2-RRDownload protocol
FC protocol for ACOX1 antibody 83731-2-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols