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GBP1 Recombinant antibody

GBP1 Uni-rAbTM Recombinant Antibody for WB, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

240634G8

Cat no : 83751-6-RR

Print datasheet

Synonyms

Guanine nucleotide-binding protein 1, GTP-binding protein 1, GBP-1, GBP 1, EC:3.6.1.-

验证数据展示

  • WB analysis using 83751-6-RR

    WB analysis using 83751-6-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 83751-6-RR (GBP1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • FC experiment of HeLa using 83751-6-RR

    FC experiment of HeLa using 83751-6-RR

    1x10^6 HeLa cells untreated (dashed lines) or treated with IFN gamma (red) were intracellularly stained with 0.25 ug GBP1 Recombinant antibody (83751-6-RR, Clone: 240634G8) and APC-Conjugated Goat Anti-Rabbit IgG(H+L), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • Affinity and Kinetic Characterization of 83751-6-RR

    Affinity and Kinetic Characterization of 83751-6-RR

    Biolayer interferometry (BLl) kinetic assays of 83751-6-RR against Human GBP1 were performed. The affinity constant is below 1 pM.

经过测试的应用

Positive WB detected inA2780 cells, HUVEC cells
Positive FC (Intra) detected inIFN gamma (100ng/ml 24h) HeLa cells
Planning an IHC experiment? We recommend our IHCeasy GBP1 Ready-To-Use IHC Kit. GBP1 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

The immunogen of 83751-6-RR is GBP1 Fusion Protein expressed in E. coli.

Tested Applications WB, FC (Intra), ELISA Application Description
Tested Reactivity human
Immunogen GBP1 fusion protein Ag7562 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name GTP binding protein 1
Synonyms Guanine nucleotide-binding protein 1, GTP-binding protein 1, GBP-1, GBP 1, EC:3.6.1.-
Calculated Molecular Weight 68 kDa
Observed Molecular Weight 67 kDa
GenBank Accession NumberBC002666
Gene Symbol GBP1
Gene ID (NCBI) 2633
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDP32455
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Guanylate binding protein 1 (GBP1) belongs to the dynamin superfamily of large GTPases. The expression of GBP1 is induced by interferon and GBP1 is characterized by its ability to specifically bind guanine nucleotides such as GMP, GDP, and GTP and its ability to hydrolyze GTP to GDP and GMP. GBP1 is induced in response to type I and type II interferons and as such plays a role in protective immunity against a spectrum of intracellular pathogens ranging from viruses to bacteria to protozoa, such as negative-strand RNA Rhabdovirus, vesicular stomatitis virus, and the positive-strand RNA Picornovirus, encephalomyocarditis virus in cultured cells, the inhibition of Chlamydia trachomatis, Toxoplasma gondii, and Salmonella enterica.

实验方案

Product Specific Protocols
WB protocol for GBP1 antibody 83751-6-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols