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验证数据展示

  • WB analysis using 83761-5-RR

    WB analysis using 83761-5-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 83761-5-RR (PAK4 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • FC experiment of MCF-7 using 83761-5-RR

    FC experiment of MCF-7 using 83761-5-RR

    1x10^6 MCF-7 cells were intracellularly stained with 0.25 ug PAK4 Recombinant antibody (83761-5-RR, Clone:240686D1) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • Affinity and Kinetic Characterization of 83761-5-RR

    Affinity and Kinetic Characterization of 83761-5-RR

    Biolayer interferometry (BLl) kinetic assays of 83761-5-RR against Human PAK4 were performed. The affinity constant is below 1 pM.

PAK4 Recombinant antibody

PAK4 Uni-rAbTM Recombinant Antibody for WB, FC (Intra), ELISA
Cat No. 83761-5-RR

产品说明书

CloneNo. 240686D1

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, FC (Intra), ELISA

别名

PAK 4, p21-activated kinase 4, p21 activated kinase 4, KIAA1142, EC:2.7.11.1

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHeLa cells, COLO 320 cells, Raji cells, MCF-7 cells, PC-3 cells
Positive FC (Intra) detected inMCF-7 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

83761-5-RR targets PAK4 in WB, FC (Intra), ELISA applications and shows reactivity with human samples.

经测试应用 WB, FC (Intra), ELISA Application Description
经测试反应性 human
免疫原 PAK4 fusion protein Ag5981 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 p21 protein (Cdc42/Rac)-activated kinase 4
别名 PAK 4, p21-activated kinase 4, p21 activated kinase 4, KIAA1142, EC:2.7.11.1
计算分子量 64 kDa
观测分子量64 kDa
GenBank蛋白编号BC025282
基因名称 PAK4
Gene ID (NCBI) 10298
RRIDAB_3671357
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purfication
UNIPROT IDO96013
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

PAK4(p21-activated kinase 4) is also named as KIAA1142. It belongs to the protein kinase superfamily.PAK4 regulates cell morphology, cytoskeletal organization, and cell proliferation and migration. It can also function as an antiapoptotic protein (PMID:14517283). It has 4 isoforms produced by alternative splicing. And it can be autophosphorylated on serine residues when activated by CDC42/p21 (PMID:11278822).

实验方案

Product Specific Protocols
WB protocol for PAK4 antibody 83761-5-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols